DNA-carrying latex particles for DNA diagnosis 2. Distinction of normal and point mutant DNA using S1 nuclease

M. Hatakeyama; K. Nakamura; S. Iwato; H. Handa; K. Fujimoto; H. Kawaguchi

doi:10.1016/S0927-7765(97)00058-1

DNA-carrying latex particles for DNA diagnosis 2. Distinction of normal and point mutant DNA using S1 nuclease

M. Hatakeyama, K. Nakamura, S. Iwato, H. Handa, K. Fujimoto, H. Kawaguchi

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

In our previous paper, we reported a DNA diagnosis system in which fully complementary DNA was selectively hybridized with a DNA immobilized on latex particles but one-point mutant DNA was not. The system, however, included a few disadvantages. To overcome them, a single-strand DNA cleaving enzyme (S1 nuclease) was used in this study. S1 nuclease cleaved the unhybridized and loosely hybridized DNA's on the particles and enabled the determination of the fully complementary hybrid. This method saved us a troublesome labeling of the samples.

Original language	English
Pages (from-to)	171-178
Number of pages	8
Journal	Colloids and Surfaces B: Biointerfaces
Volume	10
Issue number	3
DOIs	https://doi.org/10.1016/S0927-7765(97)00058-1
Publication status	Published - 1998 Feb 15

Keywords

DNA diagnosis
Microsphere
Miss-matched DNA
Point-mutation
S1 nuclease

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/S0927-7765(97)00058-1

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title = "DNA-carrying latex particles for DNA diagnosis 2. Distinction of normal and point mutant DNA using S1 nuclease",

abstract = "In our previous paper, we reported a DNA diagnosis system in which fully complementary DNA was selectively hybridized with a DNA immobilized on latex particles but one-point mutant DNA was not. The system, however, included a few disadvantages. To overcome them, a single-strand DNA cleaving enzyme (S1 nuclease) was used in this study. S1 nuclease cleaved the unhybridized and loosely hybridized DNA's on the particles and enabled the determination of the fully complementary hybrid. This method saved us a troublesome labeling of the samples.",

keywords = "DNA diagnosis, Microsphere, Miss-matched DNA, Point-mutation, S1 nuclease",

author = "M. Hatakeyama and K. Nakamura and S. Iwato and H. Handa and K. Fujimoto and H. Kawaguchi",

note = "Funding Information: This work was supportedb y the Grant-in-Aid for ScientificR esearcho n Priority Areas, {"}New Polymersa nd Their Nano-OrganizedS ystems{"} (no. 277/08246246)f rom the ministry of Education, Science, Sports and Culture, the ResearchG rant from Keio University,a nd the SasakawaS cientific ResearchG rant from the Japan ScienceS ociety.T he authorsa cknowledge themg ratefully.",

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doi = "10.1016/S0927-7765(97)00058-1",

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TY - JOUR

T1 - DNA-carrying latex particles for DNA diagnosis 2. Distinction of normal and point mutant DNA using S1 nuclease

AU - Hatakeyama, M.

AU - Nakamura, K.

AU - Iwato, S.

AU - Handa, H.

AU - Fujimoto, K.

AU - Kawaguchi, H.

N1 - Funding Information: This work was supportedb y the Grant-in-Aid for ScientificR esearcho n Priority Areas, "New Polymersa nd Their Nano-OrganizedS ystems" (no. 277/08246246)f rom the ministry of Education, Science, Sports and Culture, the ResearchG rant from Keio University,a nd the SasakawaS cientific ResearchG rant from the Japan ScienceS ociety.T he authorsa cknowledge themg ratefully.

PY - 1998/2/15

Y1 - 1998/2/15

N2 - In our previous paper, we reported a DNA diagnosis system in which fully complementary DNA was selectively hybridized with a DNA immobilized on latex particles but one-point mutant DNA was not. The system, however, included a few disadvantages. To overcome them, a single-strand DNA cleaving enzyme (S1 nuclease) was used in this study. S1 nuclease cleaved the unhybridized and loosely hybridized DNA's on the particles and enabled the determination of the fully complementary hybrid. This method saved us a troublesome labeling of the samples.

AB - In our previous paper, we reported a DNA diagnosis system in which fully complementary DNA was selectively hybridized with a DNA immobilized on latex particles but one-point mutant DNA was not. The system, however, included a few disadvantages. To overcome them, a single-strand DNA cleaving enzyme (S1 nuclease) was used in this study. S1 nuclease cleaved the unhybridized and loosely hybridized DNA's on the particles and enabled the determination of the fully complementary hybrid. This method saved us a troublesome labeling of the samples.

KW - DNA diagnosis

KW - Microsphere

KW - Miss-matched DNA

KW - Point-mutation

KW - S1 nuclease

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JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

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ER -

DNA-carrying latex particles for DNA diagnosis 2. Distinction of normal and point mutant DNA using S1 nuclease

Abstract

Keywords

ASJC Scopus subject areas

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