TY - JOUR
T1 - DNA Hybridization Assay Using Gold Nanoparticles and Electrophoresis Separation Provides 1 pM Sensitivity
AU - Esashika, Keiko
AU - Saiki, Toshiharu
N1 - Funding Information:
We are grateful to Tanaka Kikinzoku Kogyo for providing gold nanoparticles for this study. This work was supported by JSPS KAKENHI Grant Number 16K13645 and partially by the Advanced Photon Science Alliance Project from MEXT. A part of this work was supported by Advanced Characterization Nanotechnology Platform, Nanotechnology Platform Program of MEXT at the Research Center for Ultra-High Voltage Electron Microscopy (Nanotechnology Open Facilities) in Osaka University.
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2018/1/17
Y1 - 2018/1/17
N2 - The efficiency of gold nanoparticle (AuNP) dimerization mediated by hybridization between two probe DNA molecules and a complementary target DNA molecule was maximized by examining several possible hybridization combinations. The uniformity of the size of the AuNPs, the use of surface modification appropriate for high hybridization efficiency, together with efficient blocking of nonspecific binding, all contributed to achieving a 1 pM detection limit following conventional gel electrophoresis separation of the DNA-modified AuNP multimers. This practical homogeneous DNA hybridization assay methodology will provide a rapid, cost-effective, and field-portable tool for clinical diagnosis.
AB - The efficiency of gold nanoparticle (AuNP) dimerization mediated by hybridization between two probe DNA molecules and a complementary target DNA molecule was maximized by examining several possible hybridization combinations. The uniformity of the size of the AuNPs, the use of surface modification appropriate for high hybridization efficiency, together with efficient blocking of nonspecific binding, all contributed to achieving a 1 pM detection limit following conventional gel electrophoresis separation of the DNA-modified AuNP multimers. This practical homogeneous DNA hybridization assay methodology will provide a rapid, cost-effective, and field-portable tool for clinical diagnosis.
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U2 - 10.1021/acs.bioconjchem.7b00682
DO - 10.1021/acs.bioconjchem.7b00682
M3 - Article
C2 - 29200266
AN - SCOPUS:85040708112
SN - 1043-1802
VL - 29
SP - 182
EP - 189
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
IS - 1
ER -