TY - JOUR
T1 - DNA hypermethylation at the D17S5 locus is associated with gastric carcinogenesis
AU - Kanai, Yae
AU - Ushijima, Saori
AU - Ochiai, Atsushi
AU - Eguchi, Keisuke
AU - Hui, Ai Min
AU - Hirohashi, Setsuo
N1 - Funding Information:
This study was supported by a Grant-in-Aid for the Second Term Comprehensive 10-Year Strategy for Cancer Control and a Grant-in-Aid for Cancer Research from the Ministry of Health and Welfare of Japan. K.E. is a recipient of a Research Resident Fellowship from the Foundation for Promotion of Cancer Research, Tokyo. A.-M.H. is a recipient of a National Institute Postdoctoral Fellowship from the Research Development Corporation of Japan.
PY - 1998/1/9
Y1 - 1998/1/9
N2 - The aim of this study was to elucidate the significance of aberrant DNA methylation in gastric carcinogenesis. The DNA methylation status at the D17S5 locus, at which a candidate tumor suppressor gene, HIC-1, was identified, of gastric cancers and non-cancerous gastric mucosae from 42 gastric cancer patients was examined by Southern blotting using a methylation-sensitive restriction enzyme. DNA hypermethylation was observed in 15, 13, 25 and 45% of the tissues showing no remarkable histological findings, chronic gastritis without intestinal metaplasia, intestinal metaplasia and gastric cancer, respectively. The incidence of DNA hypermethylation was significantly higher in gastric cancers than in non-cancerous gastric mucosae (P < 0.05). DNA hypermethylation was often accompanied by allelic loss at the same locus in gastric cancers. DNA hypermethylation at the D17S5 locus, which was even detected in precancerous conditions, including intestinal metaplasia, may play a role in gastric carcinogenesis.
AB - The aim of this study was to elucidate the significance of aberrant DNA methylation in gastric carcinogenesis. The DNA methylation status at the D17S5 locus, at which a candidate tumor suppressor gene, HIC-1, was identified, of gastric cancers and non-cancerous gastric mucosae from 42 gastric cancer patients was examined by Southern blotting using a methylation-sensitive restriction enzyme. DNA hypermethylation was observed in 15, 13, 25 and 45% of the tissues showing no remarkable histological findings, chronic gastritis without intestinal metaplasia, intestinal metaplasia and gastric cancer, respectively. The incidence of DNA hypermethylation was significantly higher in gastric cancers than in non-cancerous gastric mucosae (P < 0.05). DNA hypermethylation was often accompanied by allelic loss at the same locus in gastric cancers. DNA hypermethylation at the D17S5 locus, which was even detected in precancerous conditions, including intestinal metaplasia, may play a role in gastric carcinogenesis.
KW - DNA hypermethylation
KW - Gastric cancer
KW - Intestinal metaplasia
KW - Loss of heterozygosity
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U2 - 10.1016/S0304-3835(97)00380-7
DO - 10.1016/S0304-3835(97)00380-7
M3 - Article
C2 - 9464502
AN - SCOPUS:0032498296
SN - 0304-3835
VL - 122
SP - 135
EP - 141
JO - Cancer Letters
JF - Cancer Letters
IS - 1-2
ER -
