Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution

Yusuke Miyauchi; Robert Kourist; Daisuke Uemura; Kenji Miyamoto

doi:10.1039/c1cc11953b

Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution

Yusuke Miyauchi, Robert Kourist, Daisuke Uemura, Kenji Miyamoto

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).

Original language	English
Pages (from-to)	7503-7505
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	26
DOIs	https://doi.org/10.1039/c1cc11953b
Publication status	Published - 2011 Jul 14

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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abstract = "Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).",

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AU - Miyamoto, Kenji

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AB - Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).

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Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution

Abstract

ASJC Scopus subject areas

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