Drosophila big brain does not act as a water channel, but mediates cell adhesion

Kimiko Tatsumi; Shoji Tsuji; Hideki Miwa; Toshinori Morisaku; Mutsuo Nuriya; Minako Orihara; Kazunari Kaneko; Hideyuki Okano; Masato Yasui

doi:10.1016/j.febslet.2009.05.035

Drosophila big brain does not act as a water channel, but mediates cell adhesion

Kimiko Tatsumi
, Shoji Tsuji
, Hideki Miwa
, Toshinori Morisaku
, Mutsuo Nuriya
, Minako Orihara
, Kazunari Kaneko
, Hideyuki Okano
, Masato Yasui

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay using L cells. Bib-transfected L cells formed aggregated clusters, while control-L cells remained as a single cell suspension. Ion permeation was not confirmed in L cells stably expressing Bib. When expressed in COS7 cells, Bib exhibited limited water permeability. This newly found cell adhesive function of Bib may be important for Drosophila neurogenesis.

Original language	English
Pages (from-to)	2077-2082
Number of pages	6
Journal	FEBS Letters
Volume	583
Issue number	12
DOIs	https://doi.org/10.1016/j.febslet.2009.05.035
Publication status	Published - 2009 Jun 18

Keywords

Aquaporin-4 (AQP4)
Big brain
Cell adhesion
Drosophila

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2009.05.035

Cite this

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title = "Drosophila big brain does not act as a water channel, but mediates cell adhesion",

abstract = "The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay using L cells. Bib-transfected L cells formed aggregated clusters, while control-L cells remained as a single cell suspension. Ion permeation was not confirmed in L cells stably expressing Bib. When expressed in COS7 cells, Bib exhibited limited water permeability. This newly found cell adhesive function of Bib may be important for Drosophila neurogenesis.",

keywords = "Aquaporin-4 (AQP4), Big brain, Cell adhesion, Drosophila",

author = "Kimiko Tatsumi and Shoji Tsuji and Hideki Miwa and Toshinori Morisaku and Mutsuo Nuriya and Minako Orihara and Kazunari Kaneko and Hideyuki Okano and Masato Yasui",

note = "Funding Information: We thank Akihiro Hazama for fruitful discussion. This work was supported by Specially Promoted Research from MEXT (Ministry of Education, Culture, Sports, Science and Technology) of Japan, in part by a Grant for Child Health and Development from the Ministry of Health, Labor and Welfare of Japan.",

year = "2009",

month = jun,

day = "18",

doi = "10.1016/j.febslet.2009.05.035",

language = "English",

volume = "583",

pages = "2077--2082",

journal = "FEBS Letters",

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TY - JOUR

T1 - Drosophila big brain does not act as a water channel, but mediates cell adhesion

AU - Tatsumi, Kimiko

AU - Tsuji, Shoji

AU - Miwa, Hideki

AU - Morisaku, Toshinori

AU - Nuriya, Mutsuo

AU - Orihara, Minako

AU - Kaneko, Kazunari

AU - Okano, Hideyuki

AU - Yasui, Masato

N1 - Funding Information: We thank Akihiro Hazama for fruitful discussion. This work was supported by Specially Promoted Research from MEXT (Ministry of Education, Culture, Sports, Science and Technology) of Japan, in part by a Grant for Child Health and Development from the Ministry of Health, Labor and Welfare of Japan.

PY - 2009/6/18

Y1 - 2009/6/18

N2 - The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay using L cells. Bib-transfected L cells formed aggregated clusters, while control-L cells remained as a single cell suspension. Ion permeation was not confirmed in L cells stably expressing Bib. When expressed in COS7 cells, Bib exhibited limited water permeability. This newly found cell adhesive function of Bib may be important for Drosophila neurogenesis.

AB - The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay using L cells. Bib-transfected L cells formed aggregated clusters, while control-L cells remained as a single cell suspension. Ion permeation was not confirmed in L cells stably expressing Bib. When expressed in COS7 cells, Bib exhibited limited water permeability. This newly found cell adhesive function of Bib may be important for Drosophila neurogenesis.

KW - Aquaporin-4 (AQP4)

KW - Big brain

KW - Cell adhesion

KW - Drosophila

UR - https://www.scopus.com/pages/publications/67349176604

UR - https://www.scopus.com/pages/publications/67349176604#tab=citedBy

U2 - 10.1016/j.febslet.2009.05.035

DO - 10.1016/j.febslet.2009.05.035

M3 - Article

C2 - 19467350

AN - SCOPUS:67349176604

SN - 0014-5793

VL - 583

SP - 2077

EP - 2082

JO - FEBS Letters

JF - FEBS Letters

IS - 12

ER -

Drosophila big brain does not act as a water channel, but mediates cell adhesion

Abstract

Keywords

ASJC Scopus subject areas

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