TY - JOUR
T1 - Dynamic change of cells expressing MMP-2 mRNA and MT1-MMP mRNA in the recovery from liver fibrosis in the rat
AU - Watanabe, Tetsu
AU - Niioka, Maki
AU - Ishikawa, Akiko
AU - Hozawa, Shigenari
AU - Arai, Masao
AU - Maruyama, Katsuya
AU - Okada, Akiko
AU - Okazaki, Isao
N1 - Funding Information:
The authors thank Ms. Sawako Yuasa and Ms. Kaori Shimosakai for excellent technical assistance. This study was supported in part by a grant-in-aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (I.O.).
PY - 2001
Y1 - 2001
N2 - Background/Aims: The aim of this study was to investigate whether both matrix metalloproteinase-2 (MMP-2) and membrane type 1 MMP (MT1-MMP) participate in the spontaneous resolution of liver fibrosis. Methods: Transcription of both genes was examined by reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH). Gelatinase activity was investigated by zymography. Results: Gene expression by RT-PCR showed that both genes increased in the process of liver fibrosis, then decreased gradually in the recovery phase. ISH revealed that distribution of positive cells changed quickly in the recovery phase. Positive cells were widely seen in the liver, mainly around fibrous septa, in the aggressive phase, but were exclusively observed at the interface between the resolving fibrous band and the parenchyma, then were diffusely located in the lobules in the recovery phase. Main cells expressing both mRNAs seemed to be stellate cells for their morphology, though they did not express characteristic cell markers. Some hepatocytes and Kupffer cells expressed both mRNAs in the recovery phase. Gelatinase activity of MMP-2 increased in the recovery phase of 8-week-treated rat liver by gelatin zymography. Conclusions: The results of present study suggest that both enzymes participate in the destruction of extracellular matrix in coordination with MMP-13.
AB - Background/Aims: The aim of this study was to investigate whether both matrix metalloproteinase-2 (MMP-2) and membrane type 1 MMP (MT1-MMP) participate in the spontaneous resolution of liver fibrosis. Methods: Transcription of both genes was examined by reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH). Gelatinase activity was investigated by zymography. Results: Gene expression by RT-PCR showed that both genes increased in the process of liver fibrosis, then decreased gradually in the recovery phase. ISH revealed that distribution of positive cells changed quickly in the recovery phase. Positive cells were widely seen in the liver, mainly around fibrous septa, in the aggressive phase, but were exclusively observed at the interface between the resolving fibrous band and the parenchyma, then were diffusely located in the lobules in the recovery phase. Main cells expressing both mRNAs seemed to be stellate cells for their morphology, though they did not express characteristic cell markers. Some hepatocytes and Kupffer cells expressed both mRNAs in the recovery phase. Gelatinase activity of MMP-2 increased in the recovery phase of 8-week-treated rat liver by gelatin zymography. Conclusions: The results of present study suggest that both enzymes participate in the destruction of extracellular matrix in coordination with MMP-13.
KW - Hepatic stellate cell
KW - In situ hybridization
KW - Matrix metalloproteinase
KW - Matrix metalloproteinase 2
KW - Membrane type 1 matrix metalloproteinase
KW - Reverse transcription-polymerase chain reaction
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U2 - 10.1016/S0168-8278(01)00177-5
DO - 10.1016/S0168-8278(01)00177-5
M3 - Article
C2 - 11682030
AN - SCOPUS:0034793038
SN - 0168-8278
VL - 35
SP - 465
EP - 473
JO - Journal of Hepatology
JF - Journal of Hepatology
IS - 4
ER -