E-cadherin is involved in the intrahepatic metastasis of hepatocellular carcinoma

Takuya Osada, Michiie Sakamoto, Yoshinori Ino, Akihiro Iwamatsu, Yoshihiro Matsuno, Tetsuichiro Muto, Setsuo Hirohashi

Research output: Contribution to journalArticlepeer-review

117 Citations (Scopus)


In human hepatocellular carcinoma (HCC), the liver is the major target organ of metastasis, which is known as intrahepatic metastasis. To analyze the mechanism of this metastasis, we established two sublines from the human HCC cell line Li7. Subline Li7HM produced multiple liver metastasis, whereas subline Li7NM never did so after intrasplenic injection into nude mice. Two- dimensional gel electrophoresis and immunoblot analysis showed that only Li7NM expressed vimentin and lacked E-cadherin expression, indicating that this clone had undergone epithelial-mesenchymal transition. We then transfected mouse E-cadherin complementary DNA into Li7NM cells and found that the transfectant cells (EM16.21B.3) formed liver metastasis (8/16 mice) after intrasplenic injection and liver tumors (11/13 mice) after intrahepatic injection, whereas the control cell line formed no tumors. These results suggest that E-cadherin plays an important role in the process of intrahepatic metastasis of HCC.

Original languageEnglish
Pages (from-to)1460-1467
Number of pages8
Issue number6
Publication statusPublished - 1996 Dec
Externally publishedYes

ASJC Scopus subject areas

  • Hepatology


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