TY - JOUR
T1 - Effect of polymer containing a naphthyl-alanine derivative on the separation selectivity for aromatic compounds in temperature-responsive chromatography
AU - Nishio, Tadashi
AU - Kanazashi, Ryosuke
AU - Nojima, Akari
AU - Kanazawa, Hideko
AU - Okano, Teruo
N1 - Funding Information:
Part of this study was supported by Grant-in-Aid for Scientific Research (B) (Grant No. 21390012 ) from Ministry of Education, Culture, Sports, Science and Technology, Japan .
PY - 2012/3/9
Y1 - 2012/3/9
N2 - A novel polymer-grafted stationary phase of high-performance liquid chromatography (HPLC) was developed, utilizing a temperature-responsive polymer containing an aromatic moiety. Firstly, we synthesized novel functional polymer poly(N-isopropylacrylamide-co-N-acryloyl-3-(2-naphthyl)- l-alanine methyl ester) [poly(NIPAAm-co-Nap)], which has temperature-responsiveness and selective retention of aromatic compounds by an intermolecular π-π interaction. The polymer exhibited a significant reversible phase transition from hydrophilic to hydrophobic in the vicinity of its lower critical solution temperature. Employing the developed polymer-grafted silica column, temperature-responsive chromatography was conducted using water as a sole mobile phase. A comparison with a conventional ODS column or a homogeneous PNIPAAm-grafted silica column showed that the retention of aromatic compounds was dramatically increased on the poly(NIPAAm-co-Nap)-grafted stationary phase. Introducing the naphthyl-alanine derivative caused a significant effect on the retention selectivity for aromatic compounds.
AB - A novel polymer-grafted stationary phase of high-performance liquid chromatography (HPLC) was developed, utilizing a temperature-responsive polymer containing an aromatic moiety. Firstly, we synthesized novel functional polymer poly(N-isopropylacrylamide-co-N-acryloyl-3-(2-naphthyl)- l-alanine methyl ester) [poly(NIPAAm-co-Nap)], which has temperature-responsiveness and selective retention of aromatic compounds by an intermolecular π-π interaction. The polymer exhibited a significant reversible phase transition from hydrophilic to hydrophobic in the vicinity of its lower critical solution temperature. Employing the developed polymer-grafted silica column, temperature-responsive chromatography was conducted using water as a sole mobile phase. A comparison with a conventional ODS column or a homogeneous PNIPAAm-grafted silica column showed that the retention of aromatic compounds was dramatically increased on the poly(NIPAAm-co-Nap)-grafted stationary phase. Introducing the naphthyl-alanine derivative caused a significant effect on the retention selectivity for aromatic compounds.
KW - Aromatic compounds
KW - Poly(N-isopropylacrylamide)
KW - Poly(N-isopropylacrylamide-co-N-acryloyl-3-(2-naphthyl)-l-alanine methyl ester)
KW - Temperature-responsive chromatography
KW - π-π interaction
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U2 - 10.1016/j.chroma.2011.07.047
DO - 10.1016/j.chroma.2011.07.047
M3 - Article
C2 - 21831387
AN - SCOPUS:84857037519
SN - 0021-9673
VL - 1228
SP - 148
EP - 154
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -