Effect of prostatic neuropeptides on invasion and migration of PC-3 prostate cancer cells

Osamu Nagakawa, Masaru Ogasawara, Hideki Fujii, Koji Murakami, Jun Murata, Hideki Fuse, Ikuo Saiki

Research output: Contribution to journalArticlepeer-review

75 Citations (Scopus)


We investigated the effect of various neuropeptides present in the prostate, including calcitonin gene-related peptide (CGRP), gastrin-releasing peptide (GRP), substance P (SP), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), calcitonin (CT), leucine-enkephalin (L-ENK), glucagon and parathyroid hormone-related protein (PTH-rP), on the invasion of PC-3 prostate cancer cells through a reconstituted basement membrane (Matrigel) using a Transwell cell culture chamber assay. Both CGRP and GRP increased the invasive capacity of tumor cells, whereas SP inhibited it. On the other hand, VIP, CT, L-ENK, NPY, glucagon and PTH-rP had no significant effect. Both CGRP and GRP also increased the haptotactic migration of tumor cells to fibronectin, but SP inhibited it. These three neuropeptides had no effect on either adhesion to fibronectin and laminin or on the gelatinolytic activities of MMP-9 in gelatin zymography, nor did they affect the growth of tumor cells at concentrations used in this study. These results indicate that both GRP and CGRP increased the invasive potential of PC-3 cells probably through enhancement of cell motility, while SP inhibited the invasiveness through suppression of motile response. Copyright (C) 1998 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)27-33
Number of pages7
JournalCancer Letters
Issue number1
Publication statusPublished - 1998 Nov 13
Externally publishedYes


  • Invasion
  • Neuropeptides
  • Prostate carcinoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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