TY - JOUR
T1 - Effect of rat CINC/gro, a member of the interleukin-8 family, on leukocytes in microcirculation of the rat mesentery
AU - Watanabe, Kazuyoshi
AU - Suematsu, Makoto
AU - Iida, Megumi
AU - Takaishi, Kiyosumi
AU - Iizuka, Yoshio
AU - Suzuki, Hidekazu
AU - Suzuki, Masayuki
AU - Tsuchiya, Masaharu
AU - Tsurufuji, Susumu
PY - 1992/2
Y1 - 1992/2
N2 - Rat cytokine-induced neutrophil chemoattractant (CINC) is a member of the IL-8 family, and its human counterpart is gro/MGSA but not IL-8. We ascertained that chemically synthesized CINC was comparable to native CINC/gro with regard to chemotactic activity for rat neutrophils and studied the effect of synthesized CINC/gro on circulating leukocytes in microvascular vessels of rat mesentery. Exposure of rat mesentery to 10-8 M authentic CINC/gro induced neutrophil adherence to and extravasation from postcapillary venules (PCVs) but not from capillaries or arterioles. CINC/gro concentrations as low as 10-10 M were effective in causing neutrophil adherence. Neutrophils adhered to thin PCVs (mean diameter, ∼25 μm) after exposure to CINC/gro for 15 min. The mean diameters of the PCV with adherence of neutrophils after exposure to CINC/gro for 30 and 60 min were 37 and 43 μm, respectively. The diameters of PCV with extravasation of neutrophils also increased in a time-dependent manner. The starting position of adherence of neutrophils was ∼25-50 μm away from the upper junction of two vessels and remained virtually unchanged during exposure to CINC/gro for 60 min. However, the distance from the start to the end of neutrophil adherence increased in a time-dependent manner. The effect of CINC/gro on adherence and extravasation of leukocytes was neutrophil specific since other leukocytes such as lymphocytes and monocytes were not identified among the adherent and extravasated leukocytes.
AB - Rat cytokine-induced neutrophil chemoattractant (CINC) is a member of the IL-8 family, and its human counterpart is gro/MGSA but not IL-8. We ascertained that chemically synthesized CINC was comparable to native CINC/gro with regard to chemotactic activity for rat neutrophils and studied the effect of synthesized CINC/gro on circulating leukocytes in microvascular vessels of rat mesentery. Exposure of rat mesentery to 10-8 M authentic CINC/gro induced neutrophil adherence to and extravasation from postcapillary venules (PCVs) but not from capillaries or arterioles. CINC/gro concentrations as low as 10-10 M were effective in causing neutrophil adherence. Neutrophils adhered to thin PCVs (mean diameter, ∼25 μm) after exposure to CINC/gro for 15 min. The mean diameters of the PCV with adherence of neutrophils after exposure to CINC/gro for 30 and 60 min were 37 and 43 μm, respectively. The diameters of PCV with extravasation of neutrophils also increased in a time-dependent manner. The starting position of adherence of neutrophils was ∼25-50 μm away from the upper junction of two vessels and remained virtually unchanged during exposure to CINC/gro for 60 min. However, the distance from the start to the end of neutrophil adherence increased in a time-dependent manner. The effect of CINC/gro on adherence and extravasation of leukocytes was neutrophil specific since other leukocytes such as lymphocytes and monocytes were not identified among the adherent and extravasated leukocytes.
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U2 - 10.1016/0014-4800(92)90023-5
DO - 10.1016/0014-4800(92)90023-5
M3 - Article
C2 - 1547869
AN - SCOPUS:0026718615
SN - 0014-4800
VL - 56
SP - 60
EP - 69
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
IS - 1
ER -