Effect of timing of collection of salivary metabolomic biomarkers on oral cancer detection

Shigeo Ishikawa, Masahiro Sugimoto, Kenichiro Kitabatake, Micheal Tu, Ayako Sugano, Iku Yamamori, Asuka Iba, Kazuyuki Yusa, Miku Kaneko, Sana Ota, Kana Hiwatari, Ayame Enomoto, Tomita Masaru, Mitsuyoshi Iino

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)


The aim of this study is to evaluate the effect of duration after meals for saliva collections for oral cancer detection using metabolomics. Saliva samples were collected from oral cancer patients (n = 22) and controls (n = 44). Saliva from cancer patients was collected 12 h after dinner, and 1.5 and 3.5 h after breakfast. Control subjects fasted >1.5 h prior to saliva collection. Hydrophilic metabolites were analyzed using capillary electrophoresis mass spectrometry. Levels of 51 metabolites differed significantly in controls vs. oral cancer patients at the 12-h fasting time point (P < 0.05). Fifteen and ten metabolites differed significantly at the 1.5- and 3.5-h time points, respectively. The area of under receiver operating characteristic curve for discriminating oral cancer patients from controls was greatest at the 12-h fasting time point. The collection time after meals affects levels of salivary metabolites for oral cancer screening. The 12-h fasting after dinner time point is optimal. This study contributes to design of saliva collection protocols for metabolomics-based biomarker discovery.

Original languageEnglish
Pages (from-to)761-770
Number of pages10
JournalAmino Acids
Issue number4
Publication statusPublished - 2017 Apr 1


  • Collection condition
  • Metabolomics
  • Oral cancer
  • Saliva

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry


Dive into the research topics of 'Effect of timing of collection of salivary metabolomic biomarkers on oral cancer detection'. Together they form a unique fingerprint.

Cite this