Effect on DNA topology by DnaA protein, the initiation factor of chromosomal DNA replication in Escherichia coli

Tohru Mizushima; Tsutomu Katayama; Kazuhisa Sekimizu

doi:10.1021/bi953088f

Effect on DNA topology by DnaA protein, the initiation factor of chromosomal DNA replication in Escherichia coli

Tohru Mizushima, Tsutomu Katayama, Kazuhisa Sekimizu

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

We examined effects on supercoiled DNA topology of DnaA protein, the initiator protein of chromosomal DNA replication in Escherichia coli. The activity was identified in an analysis of plasmid DNA incubated with DnaA protein and DNA topoisomerase I. In Superose 12 gel filtration chromatography, the activity coeluted with DnaA protein. Incubation of DnaA protein with DNA at temperatures over 24 °C was required for this activity, which was observed with either oriC plasmid or the replicative form I of φX174 with no DnaA box. As binding of ATP or ADP to DnaA protein prevented the activity of DnaA protein on DNA topology, binding of the adenine nucleotide may regulate the activity.

Original language	English
Pages (from-to)	11512-11516
Number of pages	5
Journal	Biochemistry
Volume	35
Issue number	35
DOIs	https://doi.org/10.1021/bi953088f
Publication status	Published - 1996 Sept 22

ASJC Scopus subject areas

Biochemistry

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10.1021/bi953088f

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abstract = "We examined effects on supercoiled DNA topology of DnaA protein, the initiator protein of chromosomal DNA replication in Escherichia coli. The activity was identified in an analysis of plasmid DNA incubated with DnaA protein and DNA topoisomerase I. In Superose 12 gel filtration chromatography, the activity coeluted with DnaA protein. Incubation of DnaA protein with DNA at temperatures over 24 °C was required for this activity, which was observed with either oriC plasmid or the replicative form I of φX174 with no DnaA box. As binding of ATP or ADP to DnaA protein prevented the activity of DnaA protein on DNA topology, binding of the adenine nucleotide may regulate the activity.",

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Y1 - 1996/9/22

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AB - We examined effects on supercoiled DNA topology of DnaA protein, the initiator protein of chromosomal DNA replication in Escherichia coli. The activity was identified in an analysis of plasmid DNA incubated with DnaA protein and DNA topoisomerase I. In Superose 12 gel filtration chromatography, the activity coeluted with DnaA protein. Incubation of DnaA protein with DNA at temperatures over 24 °C was required for this activity, which was observed with either oriC plasmid or the replicative form I of φX174 with no DnaA box. As binding of ATP or ADP to DnaA protein prevented the activity of DnaA protein on DNA topology, binding of the adenine nucleotide may regulate the activity.

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Effect on DNA topology by DnaA protein, the initiation factor of chromosomal DNA replication in Escherichia coli

Abstract

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