Effective cloning of unmarked DNA fragments in the bacillus subtilis 168 genome

Mitsuhiro Itaya

doi:10.1271/bbb.63.602

Effective cloning of unmarked DNA fragments in the bacillus subtilis 168 genome

Mitsuhiro Itaya

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The Bacillus subtilis 168 genome is being used to clone DNA segments, particularly unmarked DNA segments between the two available clones. To facilitate this cloning, a counter selection method was developed in which loss of a cI repressor gene rendered the host strain resistant to neomycin. This method is promoted to use the B. subtilis genome as a general cloning vehicle.

Original language	English
Pages (from-to)	602-604
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	63
Issue number	3
DOIs	https://doi.org/10.1271/bbb.63.602
Publication status	Published - 1999 Jan 1
Externally published	Yes

Keywords

Inducible promoter
Positive selection
Repressor binding

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

Access to Document

10.1271/bbb.63.602

Cite this

@article{7dcd1386000940e1b43e965f39b654da,

title = "Effective cloning of unmarked DNA fragments in the bacillus subtilis 168 genome",

abstract = "The Bacillus subtilis 168 genome is being used to clone DNA segments, particularly unmarked DNA segments between the two available clones. To facilitate this cloning, a counter selection method was developed in which loss of a cI repressor gene rendered the host strain resistant to neomycin. This method is promoted to use the B. subtilis genome as a general cloning vehicle.",

keywords = "Inducible promoter, Positive selection, Repressor binding",

author = "Mitsuhiro Itaya",

year = "1999",

month = jan,

day = "1",

doi = "10.1271/bbb.63.602",

language = "English",

volume = "63",

pages = "602--604",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "3",

}

TY - JOUR

T1 - Effective cloning of unmarked DNA fragments in the bacillus subtilis 168 genome

AU - Itaya, Mitsuhiro

PY - 1999/1/1

Y1 - 1999/1/1

N2 - The Bacillus subtilis 168 genome is being used to clone DNA segments, particularly unmarked DNA segments between the two available clones. To facilitate this cloning, a counter selection method was developed in which loss of a cI repressor gene rendered the host strain resistant to neomycin. This method is promoted to use the B. subtilis genome as a general cloning vehicle.

AB - The Bacillus subtilis 168 genome is being used to clone DNA segments, particularly unmarked DNA segments between the two available clones. To facilitate this cloning, a counter selection method was developed in which loss of a cI repressor gene rendered the host strain resistant to neomycin. This method is promoted to use the B. subtilis genome as a general cloning vehicle.

KW - Inducible promoter

KW - Positive selection

KW - Repressor binding

UR - http://www.scopus.com/inward/record.url?scp=0006919498&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0006919498&partnerID=8YFLogxK

U2 - 10.1271/bbb.63.602

DO - 10.1271/bbb.63.602

M3 - Article

AN - SCOPUS:0006919498

SN - 0916-8451

VL - 63

SP - 602

EP - 604

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 3

ER -

Effective cloning of unmarked DNA fragments in the bacillus subtilis 168 genome

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this