TY - JOUR
T1 - Effects of 3,3'4)ihydroxy-a,/j-diethylstilbene And 3,3 ',4,5'-tetrahydroxystilbene On Microtubule Assembly In Vitro, Aneuploidy Induction, And Cellular Microtubule And Actin Networks
AU - Sakakibara, Yumiko
AU - Saito, Izumi
AU - Oda, Taiko
AU - Tsujibo, Hiroshi
AU - Inamori, Yoshihiko
AU - Sato, Yoshihiro
PY - 1992/1/1
Y1 - 1992/1/1
N2 - We examined the inhibitory activities of 3,3'-dihydroxy-a,/J-diethylstilbene (DDS) and 3,3 ',4,5'-tetrahydroxystilbene (THS) on microtubule assembly in vitro and their effects on chromosome number and cellular microtubule networks in Chinese hamster V79 cells. DDS showed half the inhibitory activity of diethylstilbestrol (DES) on microtubule assembly in vitro, while THS had none of the inhibitory activity. DDS induced tetraploid at 30 /im, whereas THS was found to be inactive. Furthermore, DDS disturbed cellular microtubule networks at 100 pm. We also examined the effects of DES, DDS and THS on cellular actin networks in mouse BALB 3T3 cells. DES induced a change of actin stress fiber distribution and THS had similar activity, while DDS showed no activity.
AB - We examined the inhibitory activities of 3,3'-dihydroxy-a,/J-diethylstilbene (DDS) and 3,3 ',4,5'-tetrahydroxystilbene (THS) on microtubule assembly in vitro and their effects on chromosome number and cellular microtubule networks in Chinese hamster V79 cells. DDS showed half the inhibitory activity of diethylstilbestrol (DES) on microtubule assembly in vitro, while THS had none of the inhibitory activity. DDS induced tetraploid at 30 /im, whereas THS was found to be inactive. Furthermore, DDS disturbed cellular microtubule networks at 100 pm. We also examined the effects of DES, DDS and THS on cellular actin networks in mouse BALB 3T3 cells. DES induced a change of actin stress fiber distribution and THS had similar activity, while DDS showed no activity.
KW - 3,3',4,5/-tetrahydroxystilbene
KW - 3,3'-dihydroxy-α,β-diethylstilbene
KW - Chinese hamster V79 cell
KW - actin
KW - aneuploidy
KW - diethylstilbestrol
KW - immunofluorescence
KW - microtubule assembly
KW - mouse BALB 3T3 cell
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U2 - 10.1248/cpb.40.2115
DO - 10.1248/cpb.40.2115
M3 - Article
C2 - 1423765
AN - SCOPUS:0026786407
SN - 0009-2363
VL - 40
SP - 2115
EP - 2119
JO - Chemical and Pharmaceutical Bulletin
JF - Chemical and Pharmaceutical Bulletin
IS - 8
ER -