TY - JOUR
T1 - Effects of ambient particulate matter on a reconstructed human corneal epithelium model
AU - Ko, Ryota
AU - Hayashi, Masahiko
AU - Tanaka, Miho
AU - Okuda, Tomoaki
AU - Nishita-Hara, Chiharu
AU - Ozaki, Hiroaki
AU - Uchio, Eiichi
N1 - Funding Information:
Sample collection, preparation, and measurements were performed under the CYCLEX (CYCLone collection of PM2.5 followed by exposure experiments) project supported by the Environmental Research and Technology Development Fund (5-1651) of the Ministry of the Environment, Japan. The authors would like to thank Dr. Keiichiro HARA (Fukuoka University, Faculty of Science, Department of Earth System Science,) for sample collection under CYCLEX. This work was supported by a Grant-in-Aid for Challenging Exploratory Research (16K12611) from the Ministry of Education, Science, Sports and Culture of Japan and funds from the Academia, Industry and Government Collaborative Research organisation (Fukuoka Institute for Atmospheric Environment and Health, No. 963) of Fukuoka University.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - We evaluated the effects of ambient particulate matter (PM) on the corneal epithelium using a reconstructed human corneal epithelium (HCE) model. We collected two PM size fractions [aerodynamic diameter smaller than 2.4 µm: PM0.3–2.4 and larger than 2.4 µm: PM>2.4] and exposed these tissues to PM concentrations of 1, 10, and 100 µg/mL for 24 h. After exposure, cell viability and interleukin (IL) IL-6 and IL-8 levels were determined, and haematoxylin and eosin and immunofluorescence staining of the zonula occludens-1 (ZO-1) were performed on tissue sections. In addition, the effects of a certified reference material of urban aerosols (UA; 100 µg/mL) were also examined as a reference. The viability of cells exposed to 100 μg/mL UA and PM>2.4 decreased to 76.2% ± 7.4 and 75.4% ± 16.1, respectively, whereas PM0.3–2.4 exposure had a limited effect on cell viability. These particles did not increase IL-6 and IL-8 levels significantly even though cell viability was decreased in 100 μg/mL UA and PM>2.4. ZO-1 expression was reduced in a dose-dependent manner in all groups. Reconstructed HCE could be used as an in vitro model to study the effects of environmental PM exposure on ocular surface cell viability and inflammation.
AB - We evaluated the effects of ambient particulate matter (PM) on the corneal epithelium using a reconstructed human corneal epithelium (HCE) model. We collected two PM size fractions [aerodynamic diameter smaller than 2.4 µm: PM0.3–2.4 and larger than 2.4 µm: PM>2.4] and exposed these tissues to PM concentrations of 1, 10, and 100 µg/mL for 24 h. After exposure, cell viability and interleukin (IL) IL-6 and IL-8 levels were determined, and haematoxylin and eosin and immunofluorescence staining of the zonula occludens-1 (ZO-1) were performed on tissue sections. In addition, the effects of a certified reference material of urban aerosols (UA; 100 µg/mL) were also examined as a reference. The viability of cells exposed to 100 μg/mL UA and PM>2.4 decreased to 76.2% ± 7.4 and 75.4% ± 16.1, respectively, whereas PM0.3–2.4 exposure had a limited effect on cell viability. These particles did not increase IL-6 and IL-8 levels significantly even though cell viability was decreased in 100 μg/mL UA and PM>2.4. ZO-1 expression was reduced in a dose-dependent manner in all groups. Reconstructed HCE could be used as an in vitro model to study the effects of environmental PM exposure on ocular surface cell viability and inflammation.
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U2 - 10.1038/s41598-021-82971-1
DO - 10.1038/s41598-021-82971-1
M3 - Article
C2 - 33564109
AN - SCOPUS:85100761201
SN - 2045-2322
VL - 11
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 3417
ER -