Effects of inactivation and stimulation of locus coeruleus on respiratory activity of neonatal rat

Haruhiko Hakuno; Yoshitaka Oyamada; Michie Murai; Yoko Ito; Kazuhiro Yamaguchi

doi:10.1016/j.resp.2003.11.009

Effects of inactivation and stimulation of locus coeruleus on respiratory activity of neonatal rat

Haruhiko Hakuno
, Yoshitaka Oyamada
, Michie Murai
, Yoko Ito
, Kazuhiro Yamaguchi

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

We examined the effects of pharmacological inactivation and electrical stimulation of the locus coeruleus (LC) on the respiratory rate (RR) in isolated brainstem-spinal cord preparations of the neonatal rat. The brainstem and spinal cord were isolated en bloc from neonatal (days 1-4) Sprague-Dawley rats and superfused with artificial cerebrospinal fluid (ACSF) equilibrated with a gas mixture containing 2% CO₂ (pH 7.8). Pharmacological inactivation of the bilateral LC by means of microinjection of tetrodotoxin or noradrenaline elicited a significant decrease in RR in preparations obtained from rats aged 3-4 days, but not in preparations of rats aged 1-2 days. Stimulation of the bilateral LC with a train of electrical pulses (25-50μA, 30ms, 0.5Hz, 3-4min) caused a marginal but significant increase in RR on days 3-4, but not on days 1-2. These results indicate that the LC exerts an excitatory effect on the medullary respiratory rhythm generator in an age-dependent manner.

Original language	English
Pages (from-to)	9-18
Number of pages	10
Journal	Respiratory Physiology and Neurobiology
Volume	140
Issue number	1
DOIs	https://doi.org/10.1016/j.resp.2003.11.009
Publication status	Published - 2004 Apr 20
Externally published	Yes

Keywords

Development, respiratory rate
Mammals, rat
Mediators, noradrenaline
Nucleus, locus coeruleus
Pattern of breathing, locus coeruleus inactivation
Pharmacological agents, TTX

ASJC Scopus subject areas

General Neuroscience
Physiology
Pulmonary and Respiratory Medicine

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title = "Effects of inactivation and stimulation of locus coeruleus on respiratory activity of neonatal rat",

abstract = "We examined the effects of pharmacological inactivation and electrical stimulation of the locus coeruleus (LC) on the respiratory rate (RR) in isolated brainstem-spinal cord preparations of the neonatal rat. The brainstem and spinal cord were isolated en bloc from neonatal (days 1-4) Sprague-Dawley rats and superfused with artificial cerebrospinal fluid (ACSF) equilibrated with a gas mixture containing 2\% CO2 (pH 7.8). Pharmacological inactivation of the bilateral LC by means of microinjection of tetrodotoxin or noradrenaline elicited a significant decrease in RR in preparations obtained from rats aged 3-4 days, but not in preparations of rats aged 1-2 days. Stimulation of the bilateral LC with a train of electrical pulses (25-50μA, 30ms, 0.5Hz, 3-4min) caused a marginal but significant increase in RR on days 3-4, but not on days 1-2. These results indicate that the LC exerts an excitatory effect on the medullary respiratory rhythm generator in an age-dependent manner.",

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author = "Haruhiko Hakuno and Yoshitaka Oyamada and Michie Murai and Yoko Ito and Kazuhiro Yamaguchi",

note = "Funding Information: This study was partly funded by Keio University (Grant-in-Aid for Encouragement of Young Medical Scientists), the Ministry of Education, Science and Culture of Japan (11770027), and by Takeda Science Foundation.",

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AU - Murai, Michie

AU - Ito, Yoko

AU - Yamaguchi, Kazuhiro

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N2 - We examined the effects of pharmacological inactivation and electrical stimulation of the locus coeruleus (LC) on the respiratory rate (RR) in isolated brainstem-spinal cord preparations of the neonatal rat. The brainstem and spinal cord were isolated en bloc from neonatal (days 1-4) Sprague-Dawley rats and superfused with artificial cerebrospinal fluid (ACSF) equilibrated with a gas mixture containing 2% CO2 (pH 7.8). Pharmacological inactivation of the bilateral LC by means of microinjection of tetrodotoxin or noradrenaline elicited a significant decrease in RR in preparations obtained from rats aged 3-4 days, but not in preparations of rats aged 1-2 days. Stimulation of the bilateral LC with a train of electrical pulses (25-50μA, 30ms, 0.5Hz, 3-4min) caused a marginal but significant increase in RR on days 3-4, but not on days 1-2. These results indicate that the LC exerts an excitatory effect on the medullary respiratory rhythm generator in an age-dependent manner.

AB - We examined the effects of pharmacological inactivation and electrical stimulation of the locus coeruleus (LC) on the respiratory rate (RR) in isolated brainstem-spinal cord preparations of the neonatal rat. The brainstem and spinal cord were isolated en bloc from neonatal (days 1-4) Sprague-Dawley rats and superfused with artificial cerebrospinal fluid (ACSF) equilibrated with a gas mixture containing 2% CO2 (pH 7.8). Pharmacological inactivation of the bilateral LC by means of microinjection of tetrodotoxin or noradrenaline elicited a significant decrease in RR in preparations obtained from rats aged 3-4 days, but not in preparations of rats aged 1-2 days. Stimulation of the bilateral LC with a train of electrical pulses (25-50μA, 30ms, 0.5Hz, 3-4min) caused a marginal but significant increase in RR on days 3-4, but not on days 1-2. These results indicate that the LC exerts an excitatory effect on the medullary respiratory rhythm generator in an age-dependent manner.

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KW - Nucleus, locus coeruleus

KW - Pattern of breathing, locus coeruleus inactivation

KW - Pharmacological agents, TTX

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Effects of inactivation and stimulation of locus coeruleus on respiratory activity of neonatal rat

Abstract

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