Effects of initial passage of endotoxin through the liver on the extent of acute lung injury in a rat model

Hisato Shimada, Naoki Hasegawa, Hidefumi Koh, Sadatomo Tasaka, Mie Shimizu, Wakako Yamada, Tomoyasu Nishimura, Kazuhisa Amakawa, Mitsutomo Kono, Makoto Sawafuji, Kayoko Nakamura, Seitaro Fujishima, Kazuhiro Yamaguchi, Akitosi Ishizaka

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


We hypothesized that the extent of acute lung injury (ALI) caused by lipopolysaccharide (LPS) is modified with its initial passage through the liver. We tested this hypothesis by administering LPS, 5 mg/kg, or saline to 120 male Wistar rats via the portal vein (PV) or the inferior vena cava (IVC) over 1 h. Four experimental groups of rats were administered saline into the PV, saline into the IVC, LPS into the PV (LPS-PV group), and LPS into the IVC (LPS-IVC group), respectively. At 15 and 30 min after onset of Chromium-LPS infusion, the γ counts in the liver were higher in the LPS-PV group than that in the LPS-IVC group. The ratio of Iodine-albumin counts in lung tissue to that in plasma per unit of weight (as an assessment of pulmonary microvascular permeability) at 240 min after onset of LPS stimulation, the accumulation of polymorphonuclear cell (assessed by myeloperoxidase activity) and the concentration of tumor necrosis factor α in the lung at 60 and 240 min after onset of LPS infusion, were higher in the LPS-IVC group than in the LPS-PV group. Significant differences in several factors indicative of inflammation and in the extent of LPS-induced ALI were observed after the onset of LPS infusion, depending on whether it was delivered via the PV or the IVC. These observations suggest that the entrapping of LPS during its initial passage through the hepatic circulation may attenuate LPS-induced ALI within 4 h of initiation of LPS stimulation.

Original languageEnglish
Pages (from-to)311-315
Number of pages5
Issue number3
Publication statusPublished - 2006 Sept 1


  • Acute lung injury
  • Endotoxin
  • Inferior vena cava
  • Inflammatory cytokines
  • Lipopolysaccharide
  • Myeloperoxidase
  • Portal vein
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine


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