Efficacy of photodynamic inactivation against Pseudomonas aeruginosa with pulsed light and CW light excitation

Tohru Yamaguchi; Shunichi Sato; Satoko Kawauchi; Mitsuhiro Terakawa; Nariyoshi Shinomiya; Daizoh Saitoh; Hiroshi Ashida; Minoru Obara

doi:10.1117/12.653078

Efficacy of photodynamic inactivation against Pseudomonas aeruginosa with pulsed light and CW light excitation

Tohru Yamaguchi, Shunichi Sato, Satoko Kawauchi, Mitsuhiro Terakawa, Nariyoshi Shinomiya, Daizoh Saitoh, Hiroshi Ashida, Minoru Obara

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

We compared methylene blue (MB)-mediated photobactericidal efficacies against Pseudomonas aeruginosa when using nanosecond pulsed light and CW light. In the intensity range of 10-200 mW/cm ², there was no significant difference between two cases, while photobactericidal efficacy with nanosecond pulsed light was significantly lower than that with CW light at an intensity of 250 mW/cm ². This is attributable to the saturated absorption of MB molecules due to high peak intensity of nanosecond pulsed light. On the basis of these results, we estimated the depth dependence of bacterial killing, showing that in the skin tissue region deeper than 1.5 mm, photobactericidal efficacy with nanosecond pulsed light was higher than that with CW light. This suggests that the advantage of using high-peak-intensity pulsed light for deep tissue treatment.

Original language	English
Title of host publication	Optical Methods for Tumor Treatment and Detection
Subtitle of host publication	Mechanisms and Techniques in Photodynamic Therapy XV
DOIs	https://doi.org/10.1117/12.653078
Publication status	Published - 2006
Externally published	Yes
Event	Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV - San Jose, CA, United States Duration: 2006 Jan 21 → 2006 Jan 22

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6139
ISSN (Print)	1605-7422

Other

Other	Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV
Country/Territory	United States
City	San Jose, CA
Period	06/1/21 → 06/1/22

Keywords

Cw laser
Methylene blue
PDT
Pseudomonas aeruginosa
Pulsed laser
Saturated absorption

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

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10.1117/12.653078

Cite this

Yamaguchi, T., Sato, S., Kawauchi, S., Terakawa, M., Shinomiya, N., Saitoh, D., Ashida, H., & Obara, M. (2006). Efficacy of photodynamic inactivation against Pseudomonas aeruginosa with pulsed light and CW light excitation. In Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV Article 61390V (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6139). https://doi.org/10.1117/12.653078

Efficacy of photodynamic inactivation against Pseudomonas aeruginosa with pulsed light and CW light excitation. / Yamaguchi, Tohru; Sato, Shunichi; Kawauchi, Satoko et al.
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV. 2006. 61390V (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6139).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Yamaguchi, T, Sato, S, Kawauchi, S, Terakawa, M, Shinomiya, N, Saitoh, D, Ashida, H & Obara, M 2006, Efficacy of photodynamic inactivation against Pseudomonas aeruginosa with pulsed light and CW light excitation. in Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV., 61390V, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6139, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV, San Jose, CA, United States, 06/1/21. https://doi.org/10.1117/12.653078

Yamaguchi T, Sato S, Kawauchi S, Terakawa M, Shinomiya N, Saitoh D et al. Efficacy of photodynamic inactivation against Pseudomonas aeruginosa with pulsed light and CW light excitation. In Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV. 2006. 61390V. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.653078

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title = "Efficacy of photodynamic inactivation against Pseudomonas aeruginosa with pulsed light and CW light excitation",

abstract = "We compared methylene blue (MB)-mediated photobactericidal efficacies against Pseudomonas aeruginosa when using nanosecond pulsed light and CW light. In the intensity range of 10-200 mW/cm 2, there was no significant difference between two cases, while photobactericidal efficacy with nanosecond pulsed light was significantly lower than that with CW light at an intensity of 250 mW/cm 2. This is attributable to the saturated absorption of MB molecules due to high peak intensity of nanosecond pulsed light. On the basis of these results, we estimated the depth dependence of bacterial killing, showing that in the skin tissue region deeper than 1.5 mm, photobactericidal efficacy with nanosecond pulsed light was higher than that with CW light. This suggests that the advantage of using high-peak-intensity pulsed light for deep tissue treatment.",

keywords = "Cw laser, Methylene blue, PDT, Pseudomonas aeruginosa, Pulsed laser, Saturated absorption",

author = "Tohru Yamaguchi and Shunichi Sato and Satoko Kawauchi and Mitsuhiro Terakawa and Nariyoshi Shinomiya and Daizoh Saitoh and Hiroshi Ashida and Minoru Obara",

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doi = "10.1117/12.653078",

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AU - Yamaguchi, Tohru

AU - Sato, Shunichi

AU - Kawauchi, Satoko

AU - Terakawa, Mitsuhiro

AU - Shinomiya, Nariyoshi

AU - Saitoh, Daizoh

AU - Ashida, Hiroshi

AU - Obara, Minoru

PY - 2006

Y1 - 2006

N2 - We compared methylene blue (MB)-mediated photobactericidal efficacies against Pseudomonas aeruginosa when using nanosecond pulsed light and CW light. In the intensity range of 10-200 mW/cm 2, there was no significant difference between two cases, while photobactericidal efficacy with nanosecond pulsed light was significantly lower than that with CW light at an intensity of 250 mW/cm 2. This is attributable to the saturated absorption of MB molecules due to high peak intensity of nanosecond pulsed light. On the basis of these results, we estimated the depth dependence of bacterial killing, showing that in the skin tissue region deeper than 1.5 mm, photobactericidal efficacy with nanosecond pulsed light was higher than that with CW light. This suggests that the advantage of using high-peak-intensity pulsed light for deep tissue treatment.

AB - We compared methylene blue (MB)-mediated photobactericidal efficacies against Pseudomonas aeruginosa when using nanosecond pulsed light and CW light. In the intensity range of 10-200 mW/cm 2, there was no significant difference between two cases, while photobactericidal efficacy with nanosecond pulsed light was significantly lower than that with CW light at an intensity of 250 mW/cm 2. This is attributable to the saturated absorption of MB molecules due to high peak intensity of nanosecond pulsed light. On the basis of these results, we estimated the depth dependence of bacterial killing, showing that in the skin tissue region deeper than 1.5 mm, photobactericidal efficacy with nanosecond pulsed light was higher than that with CW light. This suggests that the advantage of using high-peak-intensity pulsed light for deep tissue treatment.

KW - Cw laser

KW - Methylene blue

KW - PDT

KW - Pseudomonas aeruginosa

KW - Pulsed laser

KW - Saturated absorption

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T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Optical Methods for Tumor Treatment and Detection

T2 - Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV

Y2 - 21 January 2006 through 22 January 2006

ER -

Efficacy of photodynamic inactivation against Pseudomonas aeruginosa with pulsed light and CW light excitation

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