Efficient production of human neutrophils from iPSCs that prevent murine lethal infection with immune cell recruitment

Masashi Miyauchi, Yusuke Ito, Fumio Nakahara, Toshiya Hino, Fumi Nakamura, Yuki Iwasaki, Taiki Kawagoshi, Junji Koya, Akihide Yoshimi, Shunya Arai, Yuki Kagoya, Mineo Kurokawa

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Neutrophils play an essential role in innate immune responses to bacterial and fungal infections, and loss of neutrophil function can increase the risk of acquiring lethal infections in clinical settings. Here, we show that engineered neutrophil-primed progenitors derived from human induced pluripotent stem cells can produce functional neutrophil-like cells at a clinically applicable scale that can act rapidly in vivo against lethal bacterial infections. Using 5 different mouse models, we systematically demonstrated that these neutrophil-like cells migrate to sites of inflammation and infection and increase survival against bacterial infection. In addition, we found that these human neutrophil-like cells can recruit murine immune cells. This system potentially provides a straight-forward solution for patients with neutrophil deficiency: an off-the-shelf neutrophil transfusion. This platform should facilitate the administration of human neutrophils for a broad spectrum of physiological and pathological conditions.

Original languageEnglish
Pages (from-to)2555-2569
Number of pages15
JournalBlood
Volume138
Issue number24
DOIs
Publication statusPublished - 2021 Dec 16
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Efficient production of human neutrophils from iPSCs that prevent murine lethal infection with immune cell recruitment'. Together they form a unique fingerprint.

Cite this