TY - JOUR
T1 - Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells
AU - Nagata, Shogo
AU - Toyoda, Masashi
AU - Yamaguchi, Shinpei
AU - Hirano, Kunio
AU - Makino, Hatsune
AU - Nishino, Koichiro
AU - Miyagawa, Yoshitaka
AU - Okita, Hajime
AU - Kiyokawa, Nobutaka
AU - Nakagawa, Masato
AU - Yamanaka, Shinya
AU - Akutsu, Hidenori
AU - Umezawa, Akihiro
AU - Tada, Takashi
PY - 2009/12
Y1 - 2009/12
N2 - Practical clinical applications for current induced pluripotent stem cell (iPSC) technologies are hindered by very low generation efficiencies. Here, we demonstrate that newborn human (h) and mouse (m) extra-embryonic amnion (AM) and yolk-sac (YS) cells, in which endogenous KLF4/. Klf4, c-MYC/c-Myc and RONIN/Ronin are expressed, can be reprogrammed to hiPSCs and miPSCs with efficiencies for AM cells of 0.02% and 0.1%, respectively. Both hiPSC and miPSCs are indistinguishable from embryonic stem cells in colony morphology, expression of pluripotency markers, global gene expression profile, DNA methylation status of OCT4 and NANOG, teratoma formation and, in the case of miPSCs, generation of germline transmissible chimeric mice. As copious amounts of human AM cells can be collected without invasion, and stored long term by conventional means without requirement for in vitro culture, they represent an ideal source for cell banking and subsequent 'on demand' generation of hiPSCs for personal regenerative and pharmaceutical applications.
AB - Practical clinical applications for current induced pluripotent stem cell (iPSC) technologies are hindered by very low generation efficiencies. Here, we demonstrate that newborn human (h) and mouse (m) extra-embryonic amnion (AM) and yolk-sac (YS) cells, in which endogenous KLF4/. Klf4, c-MYC/c-Myc and RONIN/Ronin are expressed, can be reprogrammed to hiPSCs and miPSCs with efficiencies for AM cells of 0.02% and 0.1%, respectively. Both hiPSC and miPSCs are indistinguishable from embryonic stem cells in colony morphology, expression of pluripotency markers, global gene expression profile, DNA methylation status of OCT4 and NANOG, teratoma formation and, in the case of miPSCs, generation of germline transmissible chimeric mice. As copious amounts of human AM cells can be collected without invasion, and stored long term by conventional means without requirement for in vitro culture, they represent an ideal source for cell banking and subsequent 'on demand' generation of hiPSCs for personal regenerative and pharmaceutical applications.
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U2 - 10.1111/j.1365-2443.2009.01356.x
DO - 10.1111/j.1365-2443.2009.01356.x
M3 - Article
C2 - 19912344
AN - SCOPUS:71649102793
SN - 1356-9597
VL - 14
SP - 1395
EP - 1404
JO - Genes to Cells
JF - Genes to Cells
IS - 12
ER -