Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis

M. Tabata; H. Yang; F. Mannan; Y. Katayama; T. Goda; A. Matsumoto; A. Seichi; K. Suzuki; Y. Miyahara

doi:10.1109/TRANSDUCERS.2015.7181234

Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis

M. Tabata, H. Yang, F. Mannan, Y. Katayama, T. Goda, A. Matsumoto, A. Seichi, K. Suzuki, Y. Miyahara

Department of Applied Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

Label-free miniaturized electrochemical devices have been developed to monitor the nucleic acid amplification. We successfully monitored released protons during amplification reaction in real-time manner using miniaturized pH sensors and demonstrated quantitative detection of nucleic acid. Because the sensitivity of ion-sensitive field effect transistor (ISFET) (-58.0 mV/pH) and iridium/ iridium oxide (Ir/IrOx) electrode (-57.4 mV/pH) as a pH sensor showed close values to theoretical value (-59.2 mV/pH), the combination between isothermal nucleic acid amplification and the electrochemical detection was compatible with potentially stable measurement. The proposed system would be cost-effective and portable, and potentially implemented in clinical use such as diagnosis of infectious disease and cancer.

Original language	English
Title of host publication	2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	1553-1556
Number of pages	4
ISBN (Print)	9781479989553
DOIs	https://doi.org/10.1109/TRANSDUCERS.2015.7181234
Publication status	Published - 2015 Aug 5
Event	18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States Duration: 2015 Jun 21 → 2015 Jun 25

Other

Other	18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
Country/Territory	United States
City	Anchorage
Period	15/6/21 → 15/6/25

Keywords

Electrochemical real-time monitoring
ion-sensitive field effect transistor (ISFET)
iridium/iridium oxide (Ir/IrOx) electrode
isothermal nucleic acid amplification

ASJC Scopus subject areas

Instrumentation
Electrical and Electronic Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/TRANSDUCERS.2015.7181234

Cite this

Tabata, M., Yang, H., Mannan, F., Katayama, Y., Goda, T., Matsumoto, A., Seichi, A., Suzuki, K., & Miyahara, Y. (2015). Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 (pp. 1553-1556). Article 7181234 Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2015.7181234

Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis. / Tabata, M.; Yang, H.; Mannan, F. et al.
2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. p. 1553-1556 7181234.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Tabata, M, Yang, H, Mannan, F, Katayama, Y, Goda, T, Matsumoto, A, Seichi, A, Suzuki, K & Miyahara, Y 2015, Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis. in 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015., 7181234, Institute of Electrical and Electronics Engineers Inc., pp. 1553-1556, 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Anchorage, United States, 15/6/21. https://doi.org/10.1109/TRANSDUCERS.2015.7181234

Tabata M, Yang H, Mannan F, Katayama Y, Goda T, Matsumoto A et al. Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc. 2015. p. 1553-1556. 7181234 doi: 10.1109/TRANSDUCERS.2015.7181234

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abstract = "Label-free miniaturized electrochemical devices have been developed to monitor the nucleic acid amplification. We successfully monitored released protons during amplification reaction in real-time manner using miniaturized pH sensors and demonstrated quantitative detection of nucleic acid. Because the sensitivity of ion-sensitive field effect transistor (ISFET) (-58.0 mV/pH) and iridium/ iridium oxide (Ir/IrOx) electrode (-57.4 mV/pH) as a pH sensor showed close values to theoretical value (-59.2 mV/pH), the combination between isothermal nucleic acid amplification and the electrochemical detection was compatible with potentially stable measurement. The proposed system would be cost-effective and portable, and potentially implemented in clinical use such as diagnosis of infectious disease and cancer.",

keywords = "Electrochemical real-time monitoring, ion-sensitive field effect transistor (ISFET), iridium/iridium oxide (Ir/IrOx) electrode, isothermal nucleic acid amplification",

author = "M. Tabata and H. Yang and F. Mannan and Y. Katayama and T. Goda and A. Matsumoto and A. Seichi and K. Suzuki and Y. Miyahara",

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AU - Tabata, M.

AU - Yang, H.

AU - Mannan, F.

AU - Katayama, Y.

AU - Goda, T.

AU - Matsumoto, A.

AU - Seichi, A.

AU - Suzuki, K.

AU - Miyahara, Y.

PY - 2015/8/5

Y1 - 2015/8/5

N2 - Label-free miniaturized electrochemical devices have been developed to monitor the nucleic acid amplification. We successfully monitored released protons during amplification reaction in real-time manner using miniaturized pH sensors and demonstrated quantitative detection of nucleic acid. Because the sensitivity of ion-sensitive field effect transistor (ISFET) (-58.0 mV/pH) and iridium/ iridium oxide (Ir/IrOx) electrode (-57.4 mV/pH) as a pH sensor showed close values to theoretical value (-59.2 mV/pH), the combination between isothermal nucleic acid amplification and the electrochemical detection was compatible with potentially stable measurement. The proposed system would be cost-effective and portable, and potentially implemented in clinical use such as diagnosis of infectious disease and cancer.

AB - Label-free miniaturized electrochemical devices have been developed to monitor the nucleic acid amplification. We successfully monitored released protons during amplification reaction in real-time manner using miniaturized pH sensors and demonstrated quantitative detection of nucleic acid. Because the sensitivity of ion-sensitive field effect transistor (ISFET) (-58.0 mV/pH) and iridium/ iridium oxide (Ir/IrOx) electrode (-57.4 mV/pH) as a pH sensor showed close values to theoretical value (-59.2 mV/pH), the combination between isothermal nucleic acid amplification and the electrochemical detection was compatible with potentially stable measurement. The proposed system would be cost-effective and portable, and potentially implemented in clinical use such as diagnosis of infectious disease and cancer.

KW - Electrochemical real-time monitoring

KW - ion-sensitive field effect transistor (ISFET)

KW - iridium/iridium oxide (Ir/IrOx) electrode

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Y2 - 21 June 2015 through 25 June 2015

ER -

Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis

Abstract

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Keywords

ASJC Scopus subject areas

UN SDGs

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