TY - JOUR
T1 - Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination
AU - Isobe, Keisuke
AU - Takeda, Takanori
AU - Mochizuki, Kyohei
AU - Song, Qiyuan
AU - Suda, Akira
AU - Kannari, Fumihiko
AU - Kawano, Hiroyuki
AU - Kumagai, Akiko
AU - Miyawaki, Atsushi
AU - Midorikawa, Katsumi
PY - 2013/11/1
Y1 - 2013/11/1
N2 - We demonstrate super-resolution imaging with background fluorescence rejection by interferometric temporal focusing microscopy, in which temporal focusing is combined with structured illumination. The lateral resolution and the optical sectioning capability are simultaneously improved by factors of 1.6 and 1.4, respectively, compared to conventional temporal focusing microscopy. Fluorescent beads (200 nm diameter) that are difficult to distinguish from the background fluorescence in conventional temporal focusing microscopy, are clearly visualized by interferometric temporal focusing microscopy.
AB - We demonstrate super-resolution imaging with background fluorescence rejection by interferometric temporal focusing microscopy, in which temporal focusing is combined with structured illumination. The lateral resolution and the optical sectioning capability are simultaneously improved by factors of 1.6 and 1.4, respectively, compared to conventional temporal focusing microscopy. Fluorescent beads (200 nm diameter) that are difficult to distinguish from the background fluorescence in conventional temporal focusing microscopy, are clearly visualized by interferometric temporal focusing microscopy.
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U2 - 10.1364/BOE.4.002396
DO - 10.1364/BOE.4.002396
M3 - Article
AN - SCOPUS:84887155133
SN - 2156-7085
VL - 4
SP - 2396
EP - 2410
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 11
ER -