TY - JOUR
T1 - Enhancement of optical sectioning capability of temporal focusing microscopy by using time-multiplexed multi-line focusing
AU - Inazawa, Kenta
AU - Isobe, Keisuke
AU - Ishikawa, Tomohiro
AU - Namiki, Kana
AU - Miyawaki, Atsushi
AU - Kannari, Fumihiko
AU - Midorikawa, Katsumi
N1 - Funding Information:
We are truly grateful to the Advanced Manufacturing Support Team at RIKEN Center for Advanced Photonics for manufacturing the transmission echelle grating. This work was supported by JST CREST (Grant Nos. JPMJCR1851 and JPMJCR1921), MEXT/JSPS KAKENHI (Grant No. JP18H04750 “Resonance Bio”), and the Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering grant program.
Publisher Copyright:
© 2021 The Japan Society of Applied Physics
PY - 2021/8
Y1 - 2021/8
N2 - Temporal focusing (TF) microscopy has a trade-off between temporal resolution, optical sectioning capability, and field of view. To improve this trade-off, we demonstrate time-multiplexed multi-line temporal focusing (TM-ML-TF) microscopy, which combines the time-multiplexed TF technique with multi-line focusing using a digital micromirror device. Compared with those for TF microscopy, the optical sectioning capability and the signal-to-background ratio were improved by factors of 2.5 and 4.17, respectively. In addition, by applying post-processing for structured illumination microscopy to TM-ML-TF microscopy, the optical sectioning capability and the signal-to-background ratio were further enhanced by factors of 1.2 and 4.63, respectively.
AB - Temporal focusing (TF) microscopy has a trade-off between temporal resolution, optical sectioning capability, and field of view. To improve this trade-off, we demonstrate time-multiplexed multi-line temporal focusing (TM-ML-TF) microscopy, which combines the time-multiplexed TF technique with multi-line focusing using a digital micromirror device. Compared with those for TF microscopy, the optical sectioning capability and the signal-to-background ratio were improved by factors of 2.5 and 4.17, respectively. In addition, by applying post-processing for structured illumination microscopy to TM-ML-TF microscopy, the optical sectioning capability and the signal-to-background ratio were further enhanced by factors of 1.2 and 4.63, respectively.
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U2 - 10.35848/1882-0786/ac1387
DO - 10.35848/1882-0786/ac1387
M3 - Article
AN - SCOPUS:85112070182
SN - 1882-0778
VL - 14
JO - Applied Physics Express
JF - Applied Physics Express
IS - 8
M1 - 082008
ER -