Enzymatic inactivation of major circulating forms of atrial and brain natriuretic peptides

Junko Ozaki, Hiromasa Shimizu, Yukiya Hashimoto, Hiroshi Itoh, Kazuwa Nakao, Ken Ichi Inui

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


We compared the enzymatic inactivation of major circulating forms of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP). Both ANP and BNP induced a significant increase in cyclic GMP (cGMP) formation in cultured epithelial cell line derived from porcine kidney, LLC-PK1. The cGMP formation stimulated by ANP in LLC-PK1 cells was significantly decreased by pre-treatment of the peptide with rat renal brush-border membranes, and the inactivation of ANP was inhibited by neutral endopeptidase inhibitors, phosphoramidon and S-thiorphan. BNP exhibited greater resistance to enzymatic inactivation than did ANP. In addition, phosphoramidon potentiated the natriuresis with a low dose (7.5 pmol min-1 kg-1) of ANP but not of BNP in rats. These results suggest that enzymatic degradation of natriuretic peptides is highly dependent on peptide structure, and that the affinity of BNP to neutral endopeptidase is less than that of ANP. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)307-312
Number of pages6
JournalEuropean journal of pharmacology
Issue number3
Publication statusPublished - 1999 Apr 16
Externally publishedYes


  • ANP (Atrial natriuretic peptide)
  • BNP (Brain natriuretic peptide)
  • Kidney epithelial cell line (LLC-PK)
  • Neutral endopeptidase
  • Phosphoramidon
  • Thiorphan

ASJC Scopus subject areas

  • Pharmacology


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