Epilysin (MMP-28) restrains early macrophage recruitment in Pseudomonas aeruginosa pneumonia

Anne M. Manicone, Timothy P. Birkland, Michelle Lin, Tomoko Betsuyaku, Nico Van Rooijen, Jouko Lohi, Jorma Keski-Oja, Ying Wang, Shawn J. Skerrett, William C. Parks

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77 Citations (Scopus)


Several members of the matrix metalloproteinase (MMP) family function in various processes of innate immunity, particularly in controlling leukocyte influx. Epilysin (MMP-28) is expressed in numerous tissues and, in adult mice, it has the highest expression in lung, where it is detected in bronchial epithelial cells (Clara cells). Epilysin is also expressed by bone marrow-derived macrophages, but not by alveolar macrophages, suggesting that its expression by macrophages is dependent on localization and differentiation. To assess the role of this MMP, we generated epilysin-null (Mmp28-/-) mice. Although epilysin is constitutively expressed in normal tissues, Mmp28-/- mice have no overt phenotype. However, using a murine model of Pseudomonas aeruginosa pneumonia, we found that Mmp28-/- mice had an early increase in macrophage recruitment into the lungs, as well as enhanced bacterial clearance and reduced pulmonary neutrophilia, which we predicted were due to accelerated macrophage influx. Macrophage depletion in WT and Mmp28 -/- mice confirmed a role for macrophages in clearing P. aeruginosa and regulating neutrophil recruitment. Furthermore, we observed that macrophages derived from Mmp28-/- mice migrated faster than did wildtype cells to bronchoalveolar lavage fluid from P. aeruginosa-treated mice of either genotype. These observations indicate that epilysin functions as an intrinsic negative regulator of macrophage recruitment by retarding the chemotaxis of these cells.

Original languageEnglish
Pages (from-to)3866-3876
Number of pages11
JournalJournal of Immunology
Issue number6
Publication statusPublished - 2009 Mar 15
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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