The version of this paper originally published shows incorrect units for two plasmid concentrations. In the “Reagent Setup” section, the instructions for sgRNA-Cas9 plasmid should read “Adjust the concentration of each plasmid to 1 μg μl–1,” rather than “to 1 μg ml–1.” Similarly, all concentrations in the tables in Steps 49A, 49C, and 49D should be in μg μl–1 instead of μg ml–1. Please note that these units have not been corrected in the PDF and HTML versions of the protocol available online.
ASJC Scopus subject areas
- General Biochemistry,Genetics and Molecular Biology