TY - JOUR
T1 - Establishing an induced pluripotent stem cell line from neonatal common marmoset fibroblasts by an all-in-one episomal vector approach
AU - Yoshimatsu, Sho
AU - Qian, Emi
AU - Sato, Tsukika
AU - Yamamoto, Masafumi
AU - Ishikawa, Mitsuru
AU - Okano, Hideyuki
N1 - Funding Information:
We especially thank Dr. Takashi Sasaki (Keio University) for technical support for the transcriptomic analysis, Ms. Kanae Ohtsu (Keio University) for assisting MEF and reagent preparation. We also thank all the laboratory members of H.O. for their encouragement and generous support. Data shown in this study were obtained in the “Construction of System for Spread of Primate Model Animals” project under the Strategic Research Program for Brain Sciences and Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) of the MEXT and the AMED (Grant ID: JP20dm0207001 and JP21dm0207001 to H.O.). This study was also supported by JSPS KAKENHI Grant Number 19J12871 and 20K22660 (to S.Y) and internal budgets from Keio University including the Program for the Advancement of Research in Core Projects on Longevity of the Keio University Global Research Institute from Keio University (to H.O.).
Funding Information:
We especially thank Dr. Takashi Sasaki (Keio University) for technical support for the transcriptomic analysis, Ms. Kanae Ohtsu (Keio University) for assisting MEF and reagent preparation. We also thank all the laboratory members of H.O. for their encouragement and generous support. Data shown in this study were obtained in the “Construction of System for Spread of Primate Model Animals” project under the Strategic Research Program for Brain Sciences and Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) of the MEXT and the AMED (Grant ID: JP20dm0207001 and JP21dm0207001 to H.O.). This study was also supported by JSPS KAKENHI Grant Number 19J12871 and 20K22660 (to S.Y) and internal budgets from Keio University including the Program for the Advancement of Research in Core Projects on Longevity of the Keio University Global Research Institute from Keio University (to H.O.).
Publisher Copyright:
© 2021
PY - 2021/5
Y1 - 2021/5
N2 - Epstein-Barr virus (EBV)-based episomal vector system enables persistent transgene expression, which is advantageous for efficient derivation of transgene-free induced pluripotent stem cells (iPSCs) without viral transduction. Here, we report establishment of an iPSC line from somatic fibroblasts of a neonatal common marmoset monkey (marmoset; Callithrix jacchus) using an all-in-one episomal vector that we newly developed. The established iPSC line, named NM-iPS, showed standard characteristics of pluripotency such as pluripotency-related marker expression, three germ layer differentiation, and normal karyotype (2n = 46). The novel iPSC line would be a useful resource for stem cell research using non-human primates.
AB - Epstein-Barr virus (EBV)-based episomal vector system enables persistent transgene expression, which is advantageous for efficient derivation of transgene-free induced pluripotent stem cells (iPSCs) without viral transduction. Here, we report establishment of an iPSC line from somatic fibroblasts of a neonatal common marmoset monkey (marmoset; Callithrix jacchus) using an all-in-one episomal vector that we newly developed. The established iPSC line, named NM-iPS, showed standard characteristics of pluripotency such as pluripotency-related marker expression, three germ layer differentiation, and normal karyotype (2n = 46). The novel iPSC line would be a useful resource for stem cell research using non-human primates.
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U2 - 10.1016/j.scr.2021.102380
DO - 10.1016/j.scr.2021.102380
M3 - Article
C2 - 34088009
AN - SCOPUS:85104973861
SN - 1873-5061
VL - 53
JO - Stem Cell Research
JF - Stem Cell Research
M1 - 102380
ER -