Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma

Yoshimasa Saito; Toshihide Muramatsu; Hidetsugu Saito

doi:10.1016/j.xpro.2019.100009

Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma

Yoshimasa Saito, Toshihide Muramatsu, Hidetsugu Saito

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

This protocol is a procedure for establishment and culture of cancer and non-cancer organoids using tissues from biliary tract carcinoma (BTC) patients. These BTC organoids can be used for various biological analyses and drug screening. One challenge in establishing and culturing BTC organoids is non-cancer cells contaminating surgically resected tumor tissues form organoids concurrently with cancer organoids. Careful validation that the established organoids are cancer-derived is important. For complete details on the use and generation of this protocol, please refer to Saito et al. (2019) in the journal Cell Reports.

Original language	English
Article number	100009
Journal	STAR Protocols
Volume	1
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2019.100009
Publication status	Published - 2020 Jun 19

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Neuroscience(all)
Immunology and Microbiology(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.xpro.2019.100009

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abstract = "This protocol is a procedure for establishment and culture of cancer and non-cancer organoids using tissues from biliary tract carcinoma (BTC) patients. These BTC organoids can be used for various biological analyses and drug screening. One challenge in establishing and culturing BTC organoids is non-cancer cells contaminating surgically resected tumor tissues form organoids concurrently with cancer organoids. Careful validation that the established organoids are cancer-derived is important. For complete details on the use and generation of this protocol, please refer to Saito et al. (2019) in the journal Cell Reports.",

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N2 - This protocol is a procedure for establishment and culture of cancer and non-cancer organoids using tissues from biliary tract carcinoma (BTC) patients. These BTC organoids can be used for various biological analyses and drug screening. One challenge in establishing and culturing BTC organoids is non-cancer cells contaminating surgically resected tumor tissues form organoids concurrently with cancer organoids. Careful validation that the established organoids are cancer-derived is important. For complete details on the use and generation of this protocol, please refer to Saito et al. (2019) in the journal Cell Reports.

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Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma

Abstract

ASJC Scopus subject areas

UN SDGs

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