@inbook{431a88bed80b44c18211b4021d2e0942,
title = "Establishment of 3D intestinal organoid cultures from intestinal stem cells",
abstract = "The intestinal epithelium is the most rapidly renewed tissue in adult mammals, and its renewal is strictly controlled by intestinal stem cells. Extensive studies using genetic models of intestinal epithelium have revealed the mechanisms underlying the self-renewal of intestinal stem cells. Exploiting this knowledge, we developed a novel 3D culture system that enables the outgrowth of intestinal Lgr5+ stem cells derived from mouse and human tissues into ever-expanding crypt–villus mini-guts, known as intestinal epithelial organoids. These organoids are maintained by the self-renewal of stem cells and give rise to all differentiated cell types of the intestinal epithelium. Once established, organoids can be cryopreserved and thawed when needed. This culture system has been widely used for studying stem cell behavior and gene function and for disease modeling.",
keywords = "Colon, Crypt isolation, Human, Intestinal stem cells, Lgr5, Mini-gut, Mouse, Organoid culture, Small intestine, Wnt signal",
author = "Shinya Sugimoto and Toshiro Sato",
note = "Funding Information: We thank the past and present members of the Sato laboratory for their continued support. S.S. was supported by the Japan Society for the Promotion of Science Research Fellowships for Young Scientists. This work was supported in part by JSPS KAKENHI Grant Number 15J00981. Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media LLC. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",
year = "2017",
doi = "10.1007/978-1-4939-7021-6_7",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "97--105",
booktitle = "Methods in Molecular Biology",
}
