Establishment of a new detection system for the dimerization of IRE1α by BiFC assay

Satoko Shinjo; Etsu Tashiro; Masaya Imoto

doi:10.1271/bbb.130107

Establishment of a new detection system for the dimerization of IRE1α by BiFC assay

Satoko Shinjo, Etsu Tashiro, Masaya Imoto

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We developed a new detection system for the activation of an endoplasmic reticulum (ER) stress sensor, inositol requiring kinase 1 α (IRE1α), by evaluating dimerization of it by bimolecular fluorescence complementation (BiFC) assay. By detecting the fluorescence derived from the reconstituted cerulean, this assay system enabled us to distinguish the activation behaviors of IRE1α as to ER stress-inducing compounds.

Original language	English
Pages (from-to)	1333-1336
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	77
Issue number	6
DOIs	https://doi.org/10.1271/bbb.130107
Publication status	Published - 2013
Externally published	Yes

Keywords

(IRE1α)
Bimolecular fluorescent complementation (BiFC) assay
Imaging
Inositol requiring kinase 1 α
Small molecule
Unfolded protein response (UPR)

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.130107

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title = "Establishment of a new detection system for the dimerization of IRE1α by BiFC assay",

abstract = "We developed a new detection system for the activation of an endoplasmic reticulum (ER) stress sensor, inositol requiring kinase 1 α (IRE1α), by evaluating dimerization of it by bimolecular fluorescence complementation (BiFC) assay. By detecting the fluorescence derived from the reconstituted cerulean, this assay system enabled us to distinguish the activation behaviors of IRE1α as to ER stress-inducing compounds.",

keywords = "(IRE1α), Bimolecular fluorescent complementation (BiFC) assay, Imaging, Inositol requiring kinase 1 α, Small molecule, Unfolded protein response (UPR)",

author = "Satoko Shinjo and Etsu Tashiro and Masaya Imoto",

note = "Funding Information: We would like to thank Dr. Atsushi Miyawaki for kindly providing cDNA of cerulean. This work is supported by Grants-in-Aid for Scientific Research (KAKENHI grant no. 23510283, to E.T.) from Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. S.S. was a research assistant for the Global COE Program for Human Metabolomic Systems Biology.",

year = "2013",

doi = "10.1271/bbb.130107",

language = "English",

volume = "77",

pages = "1333--1336",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "6",

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TY - JOUR

T1 - Establishment of a new detection system for the dimerization of IRE1α by BiFC assay

AU - Shinjo, Satoko

AU - Tashiro, Etsu

AU - Imoto, Masaya

N1 - Funding Information: We would like to thank Dr. Atsushi Miyawaki for kindly providing cDNA of cerulean. This work is supported by Grants-in-Aid for Scientific Research (KAKENHI grant no. 23510283, to E.T.) from Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. S.S. was a research assistant for the Global COE Program for Human Metabolomic Systems Biology.

PY - 2013

Y1 - 2013

N2 - We developed a new detection system for the activation of an endoplasmic reticulum (ER) stress sensor, inositol requiring kinase 1 α (IRE1α), by evaluating dimerization of it by bimolecular fluorescence complementation (BiFC) assay. By detecting the fluorescence derived from the reconstituted cerulean, this assay system enabled us to distinguish the activation behaviors of IRE1α as to ER stress-inducing compounds.

AB - We developed a new detection system for the activation of an endoplasmic reticulum (ER) stress sensor, inositol requiring kinase 1 α (IRE1α), by evaluating dimerization of it by bimolecular fluorescence complementation (BiFC) assay. By detecting the fluorescence derived from the reconstituted cerulean, this assay system enabled us to distinguish the activation behaviors of IRE1α as to ER stress-inducing compounds.

KW - (IRE1α)

KW - Bimolecular fluorescent complementation (BiFC) assay

KW - Imaging

KW - Inositol requiring kinase 1 α

KW - Small molecule

KW - Unfolded protein response (UPR)

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DO - 10.1271/bbb.130107

M3 - Article

C2 - 23748775

AN - SCOPUS:84880167237

SN - 0916-8451

VL - 77

SP - 1333

EP - 1336

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 6

ER -

Establishment of a new detection system for the dimerization of IRE1α by BiFC assay

Abstract

Keywords

ASJC Scopus subject areas

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