Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins

Hayato Fukusumi; Tomoko Shofuda; Yohei Bamba; Atsuyo Yamamoto; Daisuke Kanematsu; Yukako Handa; Keisuke Okita; Masaya Nakamura; Shinya Yamanaka; Hideyuki Okano; Yonehiro Kanemura

doi:10.1155/2016/7235757

Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins

Hayato Fukusumi, Tomoko Shofuda, Yohei Bamba, Atsuyo Yamamoto, Daisuke Kanematsu, Yukako Handa, Keisuke Okita, Masaya Nakamura, Shinya Yamanaka, Hideyuki Okano, Yonehiro Kanemura

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Human neural progenitor cells (hNPCs) have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC) clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural induction methods, an embryoid body (EB) formation-based method and an EB formation method using dual SMAD inhibitors (dSMADi). Our results showed that expandable hNPCs could be generated from hiPSC clones with diverse somatic tissue origins. The established hNPCs exhibited a mid/hindbrain-type neural identity and uniform expression of neural progenitor genes.

Original language	English
Article number	7235757
Journal	Stem Cells International
Volume	2016
DOIs	https://doi.org/10.1155/2016/7235757
Publication status	Published - 2016

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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title = "Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins",

abstract = "Human neural progenitor cells (hNPCs) have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC) clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural induction methods, an embryoid body (EB) formation-based method and an EB formation method using dual SMAD inhibitors (dSMADi). Our results showed that expandable hNPCs could be generated from hiPSC clones with diverse somatic tissue origins. The established hNPCs exhibited a mid/hindbrain-type neural identity and uniform expression of neural progenitor genes.",

author = "Hayato Fukusumi and Tomoko Shofuda and Yohei Bamba and Atsuyo Yamamoto and Daisuke Kanematsu and Yukako Handa and Keisuke Okita and Masaya Nakamura and Shinya Yamanaka and Hideyuki Okano and Yonehiro Kanemura",

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doi = "10.1155/2016/7235757",

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journal = "Stem Cells International",

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AU - Fukusumi, Hayato

AU - Shofuda, Tomoko

AU - Bamba, Yohei

AU - Yamamoto, Atsuyo

AU - Kanematsu, Daisuke

AU - Handa, Yukako

AU - Okita, Keisuke

AU - Nakamura, Masaya

AU - Yamanaka, Shinya

AU - Okano, Hideyuki

AU - Kanemura, Yonehiro

PY - 2016

Y1 - 2016

N2 - Human neural progenitor cells (hNPCs) have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC) clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural induction methods, an embryoid body (EB) formation-based method and an EB formation method using dual SMAD inhibitors (dSMADi). Our results showed that expandable hNPCs could be generated from hiPSC clones with diverse somatic tissue origins. The established hNPCs exhibited a mid/hindbrain-type neural identity and uniform expression of neural progenitor genes.

AB - Human neural progenitor cells (hNPCs) have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC) clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural induction methods, an embryoid body (EB) formation-based method and an EB formation method using dual SMAD inhibitors (dSMADi). Our results showed that expandable hNPCs could be generated from hiPSC clones with diverse somatic tissue origins. The established hNPCs exhibited a mid/hindbrain-type neural identity and uniform expression of neural progenitor genes.

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Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins

Abstract

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