TY - JOUR
T1 - Establishment of induced pluripotent stem cells from common marmoset fibroblasts by RNA-based reprogramming
AU - Nakajima, Mayutaka
AU - Yoshimatsu, Sho
AU - Sato, Tsukika
AU - Nakamura, Mari
AU - Okahara, Junko
AU - Sasaki, Erika
AU - Shiozawa, Seiji
AU - Okano, Hideyuki
N1 - Funding Information:
We greatly thank Takashi Sasaki (Keio University) and Tsukasa Sanosaka (Keio University) for kind supports and technical advices for transcriptomic analysis. We also thank all the laboratory members of H.O. for encouragement and generous supports for this study. Portions of this study were the result of the “Construction of System for Spread of Primate Model Animals”, which was performed under the Strategic Research Program for Brain Sciences and Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) of the MEXT and the AMED under the grant number JP18dm0207002 (to H.O.), and Scientific Research in Innovative Areas, which is the MEXT Grant-in-Aid Project FY2014-2018: “Brain Protein Aging and Dementia Control” under the grant number 26117007 (to H.O.). S.Y. was financially supported by RIKEN Junior Research Associate Program .
Funding Information:
We greatly thank Takashi Sasaki (Keio University) and Tsukasa Sanosaka (Keio University) for kind supports and technical advices for transcriptomic analysis. We also thank all the laboratory members of H.O. for encouragement and generous supports for this study. Portions of this study were the result of the ?Construction of System for Spread of Primate Model Animals?, which was performed under the Strategic Research Program for Brain Sciences and Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) of the MEXT and the AMED under the grant number JP18dm0207002 (to H.O.), and Scientific Research in Innovative Areas, which is the MEXT Grant-in-Aid Project FY2014-2018: ?Brain Protein Aging and Dementia Control? under the grant number 26117007 (to H.O.). S.Y. was financially supported by RIKEN Junior Research Associate Program.
Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/8/6
Y1 - 2019/8/6
N2 - The common marmoset (marmoset; Callithrix jacchus) shows anatomical and physiological features that are in common with humans. Establishing induced pluripotent stem cells (iPSCs) from marmosets holds promise for enhancing the utility of the animal model for biomedical and preclinical studies. However, in spite of the presence of some previous reports on marmoset iPSCs, the reprogramming technology in marmosets is still under development. In particular, the efficacy of RNA-based reprogramming has not been thoroughly investigated. In this study, we attempted RNA-based reprogramming for deriving iPSCs from marmoset fibroblasts. Although we failed to derive iPSC colonies from marmoset fibroblasts by using a conventional RNA-based reprogramming method previously validated in human fibroblasts, we succeeded in deriving colony-forming cells with a modified induction medium supplemented with a novel set of small molecules. Importantly, following one-week culture of the colony-forming cells in conventional embryonic stem cell (ESC) medium, we obtained iPSCs which express endogenous pluripotent markers and show a differentiation potential into all three germ layers. Taken together, our results indicate that RNA-based reprogramming, which is valuable for deriving transgene-free iPSCs, is applicable to marmosets.
AB - The common marmoset (marmoset; Callithrix jacchus) shows anatomical and physiological features that are in common with humans. Establishing induced pluripotent stem cells (iPSCs) from marmosets holds promise for enhancing the utility of the animal model for biomedical and preclinical studies. However, in spite of the presence of some previous reports on marmoset iPSCs, the reprogramming technology in marmosets is still under development. In particular, the efficacy of RNA-based reprogramming has not been thoroughly investigated. In this study, we attempted RNA-based reprogramming for deriving iPSCs from marmoset fibroblasts. Although we failed to derive iPSC colonies from marmoset fibroblasts by using a conventional RNA-based reprogramming method previously validated in human fibroblasts, we succeeded in deriving colony-forming cells with a modified induction medium supplemented with a novel set of small molecules. Importantly, following one-week culture of the colony-forming cells in conventional embryonic stem cell (ESC) medium, we obtained iPSCs which express endogenous pluripotent markers and show a differentiation potential into all three germ layers. Taken together, our results indicate that RNA-based reprogramming, which is valuable for deriving transgene-free iPSCs, is applicable to marmosets.
KW - Induced pluripotent stem cell
KW - Marmoset
KW - Reprogramming
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U2 - 10.1016/j.bbrc.2019.05.175
DO - 10.1016/j.bbrc.2019.05.175
M3 - Article
C2 - 31178141
AN - SCOPUS:85066800173
SN - 0006-291X
VL - 515
SP - 593
EP - 599
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -