TY - JOUR
T1 - Evaluation of IS1245 LAMP in Mycobacterium avium and the influence of host-related genetic diversity on its application
AU - Akapelwa, Mwangala Lonah
AU - Kapalamula, Thoko Flav
AU - Ouchi-Aizu, Yuki
AU - Hang'ombe, Bernard Mudenda
AU - Nishiuchi, Yukiko
AU - Gordon, Stephen V.
AU - Solo, Eddie Samuneti
AU - Tamaru, Aki
AU - Nishimura, Tomoyasu
AU - Hasegawa, Naoki
AU - Morimoto, Kozo
AU - Fukushima, Yukari
AU - Suzuki, Yasuhiko
AU - Nakajima, Chie
N1 - Funding Information:
This work was supported in part by a grant from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan , for the Joint Research Program of the Research Center for Zoonosis Control, Hokkaido University to YS, and in part by Japan Agency for Medical Research and Development (AMED) under Grant Number JP20jk0210005 , JP20jm0110021 , and JP20wm0125008 to YS.
Publisher Copyright:
© 2021 Elsevier Inc.
PY - 2021/12
Y1 - 2021/12
N2 - Early detection and treatment are paramount for the timely control of Mycobacterium avium infections. Herein, we designed a LAMP assay targeting a widely used species-specific marker IS1245 for the rapid detection of M. avium and evaluated its applicability using human (n = 137) and pig (n = 91) M. avium isolates from Japan. The developed assay could detect as low as 1 genome copy of M. avium DNA within 30 minutes. All 91 (100%) M. avium isolates from pigs were detected positive while all other tested bacterial species were negative. Interestingly, among the 137 clinical M. avium isolates, 41 (30%) were undetectable with this LAMP assay as they lacked IS1245, the absence of which was revealed by PCR and whole-genome sequencing. These findings highlighted genotypic differences in M. avium strains from humans and pigs in Japan and how this diversity can influence the applicability of a detection tool across different geographic areas and hosts.
AB - Early detection and treatment are paramount for the timely control of Mycobacterium avium infections. Herein, we designed a LAMP assay targeting a widely used species-specific marker IS1245 for the rapid detection of M. avium and evaluated its applicability using human (n = 137) and pig (n = 91) M. avium isolates from Japan. The developed assay could detect as low as 1 genome copy of M. avium DNA within 30 minutes. All 91 (100%) M. avium isolates from pigs were detected positive while all other tested bacterial species were negative. Interestingly, among the 137 clinical M. avium isolates, 41 (30%) were undetectable with this LAMP assay as they lacked IS1245, the absence of which was revealed by PCR and whole-genome sequencing. These findings highlighted genotypic differences in M. avium strains from humans and pigs in Japan and how this diversity can influence the applicability of a detection tool across different geographic areas and hosts.
KW - IS1245
KW - Japan
KW - Loop-mediated isothermal amplification (LAMP)
KW - Mycobacterium avium
KW - genetic diversity
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U2 - 10.1016/j.diagmicrobio.2021.115494
DO - 10.1016/j.diagmicrobio.2021.115494
M3 - Article
C2 - 34391980
AN - SCOPUS:85112431957
SN - 0732-8893
VL - 101
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
M1 - 115494
ER -
