TY - JOUR
T1 - Evaluation of the effect of age of the younger mice on the rejuvenation of the older mice by heterochronic parabiosis
AU - Suzuki, Yushi
AU - Takaya, Kento
AU - Watanabe, Shiho
AU - Otaki, Marika
AU - Kono, Hikaru
AU - Kishi, Kazuo
N1 - Funding Information:
This work was supported Number JP 20K18422).
Funding Information:
This work was supported by JSPS KAKENHI (Grant Number JP 20K18422).
Publisher Copyright:
© 2022. Suzuki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2022
Y1 - 2022
N2 - Heterochronic parabiosis is used to study the systemic effects of aging and involves surgically connecting two animals of different ages such that they have common blood circulation. Although this technique has been prevalent for a long time, there is no scientific consensus on the age of the animals that should be used. We hypothesized that the younger the animal, the greater would be its rejuvenating effect. Hence, to test this hypothesis, we created parabiosis of 67-week-old mice with younger mice of different ages (4-week-old and 8-week-old). We evaluated the changes in appearance and the expression IL-1A, IL-6, and Cdkn2a (p16) in the liver, kidney, brain, and skin. These cytokines belong to the senescence-associated secretory phenotype (SASP) factors, and are indicators of aging. Although we did not find any significant changes in the appearance of the mice, we found statistically significant differences in some SASP factors between the liver of the 4-week-old and 8-week-old pairs. However, overall, compared to the 8-week-old mice, the 4-week-old does not exert a significantly higher rejuvenation effect on the older mice. Hence, we concluded that the rejuvenation of older mice during heterochronic parabiosis might not be affected by the exact age of the younger mice.
AB - Heterochronic parabiosis is used to study the systemic effects of aging and involves surgically connecting two animals of different ages such that they have common blood circulation. Although this technique has been prevalent for a long time, there is no scientific consensus on the age of the animals that should be used. We hypothesized that the younger the animal, the greater would be its rejuvenating effect. Hence, to test this hypothesis, we created parabiosis of 67-week-old mice with younger mice of different ages (4-week-old and 8-week-old). We evaluated the changes in appearance and the expression IL-1A, IL-6, and Cdkn2a (p16) in the liver, kidney, brain, and skin. These cytokines belong to the senescence-associated secretory phenotype (SASP) factors, and are indicators of aging. Although we did not find any significant changes in the appearance of the mice, we found statistically significant differences in some SASP factors between the liver of the 4-week-old and 8-week-old pairs. However, overall, compared to the 8-week-old mice, the 4-week-old does not exert a significantly higher rejuvenation effect on the older mice. Hence, we concluded that the rejuvenation of older mice during heterochronic parabiosis might not be affected by the exact age of the younger mice.
KW - Aging
KW - Heterochronic parabiosis
KW - Inflammatory cytokines
KW - Rejuvenating effect
KW - Senescence-associated secretory phenotype
UR - http://www.scopus.com/inward/record.url?scp=85128245565&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85128245565&partnerID=8YFLogxK
U2 - 10.18632/aging.203966
DO - 10.18632/aging.203966
M3 - Article
C2 - 35314518
AN - SCOPUS:85128245565
SN - 0002-0966
VL - 14
SP - 2507
EP - 2512
JO - Aging
JF - Aging
IS - 6
ER -