Evidence of immunostimulating lipoprotein existing in the natural lipoteichoic acid fraction

Masahito Hashimoto, Maiko Furuyashiki, Ryoko Kaseya, Yuka Fukada, Mai Akimaru, Kazue Aoyama, Toshiomi Okuno, Toshihide Tamura, Teruo Kirikae, Fumiko Kirikae, Nobutaka Eiraku, Hirofumi Morioka, Yukari Fujimoto, Koichi Fukase, Katsuhiro Takashige, Yoichiro Moriya, Shoichi Kusumoto, Yasuo Suda

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)


Lipoteichoic acid (LTA) is a cell surface glycoconjugate of gram-positive bacteria and is reported to activate the innate immune system. We previously reported that purified LTA obtained from Enterococcus hirae has no immunostimulating activity, but a subfraction (Eh-AF) in an LTA fraction possesses activity. In this study, we established a mouse monoclonal antibody neutralizing the activity of Eh-AF and investigated its inhibitory effects. Monoclonal antibody (MAbEh1) was established by the immunization of BALB/c mice with Eh-AF, followed by hybridoma screening based on its inhibitory effect for the production of interleukin-6 (IL-6) induced by Eh-AF. MAbEh1 neutralized the production of IL-6 by LTA fraction from not only E. hirae but also Staphylococcus aureus, while it failed to block that of lipopolysaccharide, suggesting that the antibody recognized a common active structure(s) in LTA fractions. Synthetic glycolipids in these LTAs did not induce cytokine production, at least in our system. Interestingly, the antibody was found to inhibit the activity of immunostimulating synthetic lipopeptides, Pam 3CSK4 and FSL-1. These results suggest that MAbEh1 neutralizes the activity of lipoprotein-like compounds which is responsible for the activity of the LTA fraction of E. hirae and S. aureus.

Original languageEnglish
Pages (from-to)1926-1932
Number of pages7
JournalInfection and Immunity
Issue number4
Publication statusPublished - 2007 Apr
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases


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