TY - JOUR
T1 - Evolutionary conservation of a unique amino acid sequence in human DICER protein essential for binding to Argonaute family proteins
AU - Sasaki, Takashi
AU - Shimizu, Nobuyoshi
N1 - Funding Information:
The authors thank T. Adachi for her excellent technical assistance. This work was supported by a Fund for “Research for the Future” Program from the Japan Society for the Promotion of Science and Grant-in-Aid for Scientific Research on Priority Areas “Medical Genome Science” from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT).
PY - 2007/7/15
Y1 - 2007/7/15
N2 - The Argonaute family and DICER proteins are major key proteins involved in the RNA-mediated gene silencing mechanism of various species. In this mechanism, cleavage of messenger RNAs (mRNA) or suppression of mRNA translation takes place via small RNAs that are uniquely processed by DICER. Previously, we demonstrated that human Argonaute family proteins bind to DICER. In this study, we identified a unique amino acid sequence of 127 amino acids in the RIBOc-A domain of human DICER protein as a "binding site" to Argonaute proteins. Comparative genomics analysis revealed that this unique amino acid sequence is highly conserved in the vertebrates, but not found in the non-vertebrate species. Significant difference in the RIBOc-A domain of DICER protein between vertebrate and non-vertebrate species may help exploring the functional complexity in the RNA-mediated gene silencing mechanism.
AB - The Argonaute family and DICER proteins are major key proteins involved in the RNA-mediated gene silencing mechanism of various species. In this mechanism, cleavage of messenger RNAs (mRNA) or suppression of mRNA translation takes place via small RNAs that are uniquely processed by DICER. Previously, we demonstrated that human Argonaute family proteins bind to DICER. In this study, we identified a unique amino acid sequence of 127 amino acids in the RIBOc-A domain of human DICER protein as a "binding site" to Argonaute proteins. Comparative genomics analysis revealed that this unique amino acid sequence is highly conserved in the vertebrates, but not found in the non-vertebrate species. Significant difference in the RIBOc-A domain of DICER protein between vertebrate and non-vertebrate species may help exploring the functional complexity in the RNA-mediated gene silencing mechanism.
KW - Protein-protein interaction
KW - RNAi
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U2 - 10.1016/j.gene.2007.04.001
DO - 10.1016/j.gene.2007.04.001
M3 - Article
C2 - 17482383
AN - SCOPUS:34250641109
SN - 0378-1119
VL - 396
SP - 312
EP - 320
JO - Gene
JF - Gene
IS - 2
ER -