Examination of the Disinfection Activity of Quick-drying Rubbing-type Antiseptics for Daily Use

Nagisa Ishiwata, Kanako Suzuki, Haruna Matsumoto, Keiko Yajima, Mariko Ono, Saori Tsuchida, Mari Hodotsuka, Mizuki Yuhara, Kayoko Maezawa, Tomoko Terajima, Noriko Kobayashi, Junko Kizu

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Hand hygiene is important as a strategy to prevent hospital infection. In particular, alcohol-containing antiseptic hand rubs (alcohol-based hand rubs) are currently essential in clinical practice due to their simplicity and potent antiseptic effects. In this study, we compared the antiseptic and prolonged effects of representative rubbing-type antiseptics routinely employed in clinical practice, such as 0.2 w/v% benzalkonium chloride alcohol preparation, 0.5 w/v% quick-drying povidone-iodine alcohol preparation, and 0.2/0.5 w/v% quick-drying chlorhexidine gluconate alcohol preparations, applied in accordance with the glove juice method at various volumes. The subjects were female pharmacy students, who operated nozzle-type containers containing these rubbing-type antiseptics to collect typical samples by pushing the nozzle, as usually used by pharmacy students for hand/finger antisepsis, and the delivered volume was measured. The bacterial reduction rate after 1, 2, or 3 mL of each preparation was rubbed into the hands/fingers 3 times. All preparations exhibited favorable antiseptic effects when the sample was 2 mL or more. In addition, the antiseptic effects persisted for 4 hours when using 2 mL of preparation 3 times. Usually, the collected volumes per push of preparations containing 0.2 w/v% benzalkonium chloride and 0.5 w/v% chlorhexidine gluconate were approximately 2 mL. However, the volumes of preparations containing 0.5 w/v% povidone-iodine and 0.2 w/v% chlorhexidine gluconate were less than 2 mL. To achieve effective hand/finger antisepsis, the volumes of antiseptics applied must be considered.

Original languageEnglish
Pages (from-to)319-324
Number of pages6
JournalJapanese Journal of Environmental Infections
Issue number5
Publication statusPublished - 2009


  • dose
  • glove juice method
  • hand/finger antisepsis
  • quick-drying rubbing-type antiseptics

ASJC Scopus subject areas

  • Epidemiology


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