TY - JOUR
T1 - Expression and characterization of Xenopus laevis SRY-related cDNAs, xSox17α1, xSox17α2, xSox18α and xSox18β
AU - Hasegawa, Masanori
AU - Hiraoka, Yoshiki
AU - Hagiudal, Jun
AU - Ogawa, Motoyuki
AU - Aiso, Sadakazu
N1 - Funding Information:
This work was supported in part by a National Grant-in-Aid for the Establishment of a High-Tech Research Center in a Private University from the Ministry of Education, Science and Culture to S.A., by Grants-in-Aid from the Ministry of Education, Science and Culture to S.A. and Y.H., by Keio Gakuji Fukuzawa Memorial Funds for Advancement of Education and Research and by Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.
PY - 2002/5/15
Y1 - 2002/5/15
N2 - Sox is a large family of genes related to the sex-determining region Y gene (designated as the SRY gene). Sox genes encoding DNA-binding transcriptional factors are found in many animals and are involved in developmental events. In this study, we newly isolated and sequenced novel Sox cDNAs from African clawed frog (Xenopus laevis). Five clones isolated here were classified into four distinct Sox genes designated as xSox17α1, xSox17α2, xSox18α and xSox18β. All four belong to a subtype of SOX family, type F. The cDNA xSox17α1 contains essentially the same nucleotide sequence as that identified as Sox17α in a previous work (Cell 91 (1997) 397), whereas xSox17α2 is a distinct gene with high homology to xSox17α1. The clones, xSox18α and xSox18β, are highly homologous to each other over the entire nucleotide sequences. The xSox18α and xSox18β genes encode 363 and 361 amino acids, respectively. Genomic Southern hybridization analysis showed the existence of two copies of the xSox18. Northern analysis indicated that the xSox18 gene was expressed in the spleen and kidney and the size of the transcript was estimated to be 2.4 knt. Electrophoretic mobility shift assays indicated that recombinant xSox18 polypeptide was capable of binding to the HMG consensus nucleotide sequence, AACAAT.
AB - Sox is a large family of genes related to the sex-determining region Y gene (designated as the SRY gene). Sox genes encoding DNA-binding transcriptional factors are found in many animals and are involved in developmental events. In this study, we newly isolated and sequenced novel Sox cDNAs from African clawed frog (Xenopus laevis). Five clones isolated here were classified into four distinct Sox genes designated as xSox17α1, xSox17α2, xSox18α and xSox18β. All four belong to a subtype of SOX family, type F. The cDNA xSox17α1 contains essentially the same nucleotide sequence as that identified as Sox17α in a previous work (Cell 91 (1997) 397), whereas xSox17α2 is a distinct gene with high homology to xSox17α1. The clones, xSox18α and xSox18β, are highly homologous to each other over the entire nucleotide sequences. The xSox18α and xSox18β genes encode 363 and 361 amino acids, respectively. Genomic Southern hybridization analysis showed the existence of two copies of the xSox18. Northern analysis indicated that the xSox18 gene was expressed in the spleen and kidney and the size of the transcript was estimated to be 2.4 knt. Electrophoretic mobility shift assays indicated that recombinant xSox18 polypeptide was capable of binding to the HMG consensus nucleotide sequence, AACAAT.
KW - DNA binding
KW - Embryogenesis
KW - HMG box
KW - Nucleotide sequence
KW - SOX
KW - SRY
KW - Xenopus laevis
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U2 - 10.1016/S0378-1119(02)00554-1
DO - 10.1016/S0378-1119(02)00554-1
M3 - Article
C2 - 12062811
AN - SCOPUS:0037094378
SN - 0378-1119
VL - 290
SP - 163
EP - 172
JO - Gene
JF - Gene
IS - 1-2
ER -