TY - JOUR
T1 - Expression and localization of aquaporin-4 in sensory ganglia
AU - Kato, Jungo
AU - Takai, Yoshiki
AU - Hayashi, Mariko Kato
AU - Kato, Yasuhiro
AU - Tanaka, Manami
AU - Sohma, Yoshiro
AU - Abe, Yoichiro
AU - Yasui, Masato
N1 - Funding Information:
We would like to thank Camilla Svensson and her colleagues for their kind technical support for the behavioral analyses and immunohistochemical analyses. The authors declare no conflicts of interests. This work was supported by the Keio Graduate School Doctral Student Grant-in-Aid Program (J.K.), Institutional Program for Young Researcher Overseas Visits from Japan Society for the Promotion of Science (J.K.), The Scandinavia-Japan Sasakawa Foundation (J.K.) and Keio University Program for the Advancement of Next Generation Research Projects (M.Y.), a Grant-in-Aid for Scientific Research on Innovative Areas for Glial assembly: a new regulatory machinery of brain function and disorders from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan (M.Y.).
Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2014/9/5
Y1 - 2014/9/5
N2 - Aquaporin-4 (AQP4) is a water channel protein that is predominantly expressed in astrocytes in the CNS. The rapid water flux through AQP4 may contribute to electrolyte/water homeostasis and may support neuronal activities in the CNS. On the other hand, little is known about the expression of AQP4 in the peripheral nervous system (PNS). Using AQP4-/- mice as a negative control, we demonstrated that AQP4 is also expressed in sensory ganglia, such as trigeminal ganglia and dorsal root ganglia in the PNS. Immunohistochemistry revealed that AQP4 is exclusively localized to satellite glial cells (SGCs) surrounding the cell bodies of the primary afferent sensory neurons in the sensory ganglia. Biochemical analyses revealed that the expression levels of AQP4 in sensory ganglia were considerably lower than those in astrocytes in the CNS. Consistently, behavioral analyses did not show any significant difference in terms of mechanical and cold sensitivity between wild type and AQP4-/- mice. Overall, although the pathophysiological relevance of AQP4 in somatosensory perception remains unclear, our findings provide new insight into the involvement of water homeostasis in the peripheral sensory system.
AB - Aquaporin-4 (AQP4) is a water channel protein that is predominantly expressed in astrocytes in the CNS. The rapid water flux through AQP4 may contribute to electrolyte/water homeostasis and may support neuronal activities in the CNS. On the other hand, little is known about the expression of AQP4 in the peripheral nervous system (PNS). Using AQP4-/- mice as a negative control, we demonstrated that AQP4 is also expressed in sensory ganglia, such as trigeminal ganglia and dorsal root ganglia in the PNS. Immunohistochemistry revealed that AQP4 is exclusively localized to satellite glial cells (SGCs) surrounding the cell bodies of the primary afferent sensory neurons in the sensory ganglia. Biochemical analyses revealed that the expression levels of AQP4 in sensory ganglia were considerably lower than those in astrocytes in the CNS. Consistently, behavioral analyses did not show any significant difference in terms of mechanical and cold sensitivity between wild type and AQP4-/- mice. Overall, although the pathophysiological relevance of AQP4 in somatosensory perception remains unclear, our findings provide new insight into the involvement of water homeostasis in the peripheral sensory system.
KW - AQP4
KW - Satellite glial cells
KW - Sensory ganglia
KW - The peripheral nervous system
KW - Water homeostasis
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U2 - 10.1016/j.bbrc.2014.08.026
DO - 10.1016/j.bbrc.2014.08.026
M3 - Article
C2 - 25124666
AN - SCOPUS:84908384198
SN - 0006-291X
VL - 451
SP - 562
EP - 567
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -