Expression of a human multidrug resistance gene in human ovarian carcinoma cell lines

S. Sekiya; T. Nunoyama; H. Shirasawa; H. Kimura; M. Kawata; N. Iijima; Y. Sugimoto; T. Tsuruo; H. Takamizawa

doi:10.1007/BF02759915

Expression of a human multidrug resistance gene in human ovarian carcinoma cell lines

S. Sekiya, T. Nunoyama, H. Shirasawa, H. Kimura, M. Kawata, N. Iijima, Y. Sugimoto, T. Tsuruo, H. Takamizawa

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

To investigate the possible role of the multidrug resistance phenotype to chemoresistance in human ovarian carcinoma, we have analyzed human multidrug resistance gene (mdr 1) expression in 8 human ovarian adenocarcinoma cell lines. An increase in P-glycoprotein level specific to multidrug-resistant tumor cells was not apparently associated with the increase in resistance to vincristine (VCR) or doxorubicin (Adriamycin). Mdr 1 transcripts (4.5 kilobases) were observed in the RNA preparation obtained from only one cell line (SHIN-3) that showed the highest resistance to both drugs in vitro and in vivo. No cell lines showed mdr 1 DNA amplification. These results suggest that the insensitivity of human ovarian carcinoma to chemotherapy could be partly explained by the expression of mdr 1.

Original language	English
Pages (from-to)	79-86
Number of pages	8
Journal	Archives of gynecology and obstetrics
Volume	251
Issue number	2
DOIs	https://doi.org/10.1007/BF02759915
Publication status	Published - 1992 Jun
Externally published	Yes

Keywords

Mdr 1 gene
Multidrug resistance
Ovarian carcinoma
P-glycoprotein

ASJC Scopus subject areas

Obstetrics and Gynaecology

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10.1007/BF02759915

Cite this

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title = "Expression of a human multidrug resistance gene in human ovarian carcinoma cell lines",

abstract = "To investigate the possible role of the multidrug resistance phenotype to chemoresistance in human ovarian carcinoma, we have analyzed human multidrug resistance gene (mdr 1) expression in 8 human ovarian adenocarcinoma cell lines. An increase in P-glycoprotein level specific to multidrug-resistant tumor cells was not apparently associated with the increase in resistance to vincristine (VCR) or doxorubicin (Adriamycin). Mdr 1 transcripts (4.5 kilobases) were observed in the RNA preparation obtained from only one cell line (SHIN-3) that showed the highest resistance to both drugs in vitro and in vivo. No cell lines showed mdr 1 DNA amplification. These results suggest that the insensitivity of human ovarian carcinoma to chemotherapy could be partly explained by the expression of mdr 1.",

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T1 - Expression of a human multidrug resistance gene in human ovarian carcinoma cell lines

AU - Sekiya, S.

AU - Nunoyama, T.

AU - Shirasawa, H.

AU - Kimura, H.

AU - Kawata, M.

AU - Iijima, N.

AU - Sugimoto, Y.

AU - Tsuruo, T.

AU - Takamizawa, H.

PY - 1992/6

Y1 - 1992/6

N2 - To investigate the possible role of the multidrug resistance phenotype to chemoresistance in human ovarian carcinoma, we have analyzed human multidrug resistance gene (mdr 1) expression in 8 human ovarian adenocarcinoma cell lines. An increase in P-glycoprotein level specific to multidrug-resistant tumor cells was not apparently associated with the increase in resistance to vincristine (VCR) or doxorubicin (Adriamycin). Mdr 1 transcripts (4.5 kilobases) were observed in the RNA preparation obtained from only one cell line (SHIN-3) that showed the highest resistance to both drugs in vitro and in vivo. No cell lines showed mdr 1 DNA amplification. These results suggest that the insensitivity of human ovarian carcinoma to chemotherapy could be partly explained by the expression of mdr 1.

AB - To investigate the possible role of the multidrug resistance phenotype to chemoresistance in human ovarian carcinoma, we have analyzed human multidrug resistance gene (mdr 1) expression in 8 human ovarian adenocarcinoma cell lines. An increase in P-glycoprotein level specific to multidrug-resistant tumor cells was not apparently associated with the increase in resistance to vincristine (VCR) or doxorubicin (Adriamycin). Mdr 1 transcripts (4.5 kilobases) were observed in the RNA preparation obtained from only one cell line (SHIN-3) that showed the highest resistance to both drugs in vitro and in vivo. No cell lines showed mdr 1 DNA amplification. These results suggest that the insensitivity of human ovarian carcinoma to chemotherapy could be partly explained by the expression of mdr 1.

KW - Mdr 1 gene

KW - Multidrug resistance

KW - Ovarian carcinoma

KW - P-glycoprotein

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Expression of a human multidrug resistance gene in human ovarian carcinoma cell lines

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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