Expression of Biologically Active Receptors for Natriuretic Peptides in the Human Uterus during Pregnancy

Hiroaki Itoh, Norimasa Sagawa, Masaaki Hasegawa, Hidetaka Nanno, Fuminori Kobayashi, Yoshiyuki Ihara, Takahide Mori, Yasato Komatsu, Shin Ichi Suga, Takaaki Yoshimasa, Hiroshi Itoh, Kazuwa Nakao

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)


Human amnion cells secrete a large amount of brain natriuretic peptide (BNP). In the present study, to elucidate the possible roles of amniotic BNP during the course of human pregnancy, the tissue distributions of biologically active receptors of natriuretic peptides, atrial natriuretic peptide (ANP)-A receptor and ANP-B receptor in the human uterus during pregnancy were investigated by Northern blot analysis and an in vitro guanosine 3′,5′-cyclic phosphate (cGMP) generation assay, the second messenger of natriuretic peptides, using crude membrane preparations. Both ANP-A and ANP-B receptor mRNAs were detected in the tissues of decidua vera, chorion laeve, myometrium and placenta. The in vitro cGMP generation assay revealed that the expression of ANP-A receptor was more prominent than that of ANP-B receptor in these tissues. On the other hand, in amniotic tissue only ANP-B receptor mRNA was weakly expressed but ANP-A receptor mRNA was not detected.

Original languageEnglish
Pages (from-to)602-607
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - 1994 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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