TY - JOUR
T1 - Expression of Biologically Active Receptors for Natriuretic Peptides in the Human Uterus during Pregnancy
AU - Itoh, Hiroaki
AU - Sagawa, Norimasa
AU - Hasegawa, Masaaki
AU - Nanno, Hidetaka
AU - Kobayashi, Fuminori
AU - Ihara, Yoshiyuki
AU - Mori, Takahide
AU - Komatsu, Yasato
AU - Suga, Shin Ichi
AU - Yoshimasa, Takaaki
AU - Itoh, Hiroshi
AU - Nakao, Kazuwa
PY - 1994/1/1
Y1 - 1994/1/1
N2 - Human amnion cells secrete a large amount of brain natriuretic peptide (BNP). In the present study, to elucidate the possible roles of amniotic BNP during the course of human pregnancy, the tissue distributions of biologically active receptors of natriuretic peptides, atrial natriuretic peptide (ANP)-A receptor and ANP-B receptor in the human uterus during pregnancy were investigated by Northern blot analysis and an in vitro guanosine 3′,5′-cyclic phosphate (cGMP) generation assay, the second messenger of natriuretic peptides, using crude membrane preparations. Both ANP-A and ANP-B receptor mRNAs were detected in the tissues of decidua vera, chorion laeve, myometrium and placenta. The in vitro cGMP generation assay revealed that the expression of ANP-A receptor was more prominent than that of ANP-B receptor in these tissues. On the other hand, in amniotic tissue only ANP-B receptor mRNA was weakly expressed but ANP-A receptor mRNA was not detected.
AB - Human amnion cells secrete a large amount of brain natriuretic peptide (BNP). In the present study, to elucidate the possible roles of amniotic BNP during the course of human pregnancy, the tissue distributions of biologically active receptors of natriuretic peptides, atrial natriuretic peptide (ANP)-A receptor and ANP-B receptor in the human uterus during pregnancy were investigated by Northern blot analysis and an in vitro guanosine 3′,5′-cyclic phosphate (cGMP) generation assay, the second messenger of natriuretic peptides, using crude membrane preparations. Both ANP-A and ANP-B receptor mRNAs were detected in the tissues of decidua vera, chorion laeve, myometrium and placenta. The in vitro cGMP generation assay revealed that the expression of ANP-A receptor was more prominent than that of ANP-B receptor in these tissues. On the other hand, in amniotic tissue only ANP-B receptor mRNA was weakly expressed but ANP-A receptor mRNA was not detected.
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U2 - 10.1006/bbrc.1994.2225
DO - 10.1006/bbrc.1994.2225
M3 - Article
C2 - 7915521
AN - SCOPUS:0027977868
SN - 0006-291X
VL - 203
SP - 602
EP - 607
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -