Abstract
We examined the expression of β 1,4-GT gene products in 11 gynecological cancer cell lines. A 4.7 kb mRNA and protein (54,000 Da and 57,000 Da) were detected by Northern blot and Western blot. Immunocytochemical staining revealed that β 1,4-GT was localized in the Golgi or ER of tumor cells. An intense β 1,4-GT mRNA signal was detected in ovarian and cervical cancer cells, whereas the level of β 1,4-GT mRNA was very low in uterine endometrial cancer cells. We also confirmed that expression of β 1,4-GT mRNA corresponded to expression of β 1,4-GT protein. These results suggest that expression of the β 1,4-GT gene products is higher in human cervical and ovarian cancer cells than in uterine endometrial cancer cells.
| Original language | English |
|---|---|
| Pages (from-to) | 117-122 |
| Number of pages | 6 |
| Journal | International journal of oncology |
| Volume | 11 |
| Issue number | 1 |
| Publication status | Published - 1997 |
Keywords
- Gynecological cancer
- Human β 1,4-galactosyltransferase
- Immunocytochemical staining
- Northern blot analysis
- Western blot analysis
- cDNA probes
ASJC Scopus subject areas
- Oncology
- Cancer Research
