Expression of membrane-type matrix metalloproteinase in human gastric carcinomas

H. Nomura, H. Sato, M. Seiki, M. Mat, Y. Okada

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305 Citations (Scopus)


We have examined the implications of membrane-type matrix metalloproteinase (MT-MMP) for activation of the zymogen of MMP-2 (proMMP-2) in human gastric carcinoma. Northern blot analysis demonstrated exclusive expression of MT-MMP in the carcinoma tissues. Immunohistochemically, MT-MMP was colocalized in the carcinoma cells in almost all MMP-2-positive cases (13 of 14 cases). Gelatin zymography of the culture media showed a correlation of the proMMP-2 activation with MT-MMP expression in the carcinoma cells. A microdissection study indicated that proMMP-2 activation is caused only in the carcinoma cell nests that express MT-MMP but not in the normal gastric mucosa. These results are the first demonstration of the MT-MMP-assisted activation of proMMP-2 in the human gastric carcinoma.

Original languageEnglish
Pages (from-to)3263-3266
Number of pages4
JournalCancer Research
Issue number15
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


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