TY - JOUR
T1 - Expression of nitric oxide synthase in a murine model of viral myocarditis induced by coxsackievirus B3
AU - Mikami, Shuji
AU - Kawashima, Seinosuke
AU - Kanazawa, Kenji
AU - Hirata, Ken Ichi
AU - Katayama, Yuko
AU - Hotta, Haku
AU - Hayashi, Yoshitake
AU - Ito, Hiroshi
AU - Yokoyama, Mitsuhiro
N1 - Funding Information:
1This study was supported by grants-in-aid for scientific research (No. 06670717) from the Ministry of Education, Science, and Culture. 2Corresponding author. Fax: +81-78-341-1390.
PY - 1996/3/27
Y1 - 1996/3/27
N2 - Nitric oxide is known to participate in the immune and inflammatory processes. In this study, we investigated the production of nitric oxide (NO) in murine viral myocarditis induced by coxsackievirus B3. The expression of inducible NO synthase (iNOS) mRNA in the heart first appeared on day 4 after virus inoculation and it was detectable for one month by reverse transcriptase-polymerase chain reaction (RT-PCR) methods. iNOS activity which was determined by the conversion of L-[3H] arginine to L-[3H]citrulline was increased on day 4 and revealed its peak on day 8. Immunohistochemistry on day 7 showed increased iNOS staining mainly in infiltrating macrophages and polymorphonuclear leukocytes. Thus, NO is thought to be produced in the heart and play an important role in a murine model of coxsackievirus B3-induced viral myocarditis.
AB - Nitric oxide is known to participate in the immune and inflammatory processes. In this study, we investigated the production of nitric oxide (NO) in murine viral myocarditis induced by coxsackievirus B3. The expression of inducible NO synthase (iNOS) mRNA in the heart first appeared on day 4 after virus inoculation and it was detectable for one month by reverse transcriptase-polymerase chain reaction (RT-PCR) methods. iNOS activity which was determined by the conversion of L-[3H] arginine to L-[3H]citrulline was increased on day 4 and revealed its peak on day 8. Immunohistochemistry on day 7 showed increased iNOS staining mainly in infiltrating macrophages and polymorphonuclear leukocytes. Thus, NO is thought to be produced in the heart and play an important role in a murine model of coxsackievirus B3-induced viral myocarditis.
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U2 - 10.1006/bbrc.1996.0519
DO - 10.1006/bbrc.1996.0519
M3 - Article
C2 - 8607880
AN - SCOPUS:0030011765
SN - 0006-291X
VL - 220
SP - 983
EP - 989
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -