Abstract
In order to examine expression of the Tn antigen on erythroid cells from a patient with Tn syndrome, we applied a selective two phase liquid culture system for human erythroid progenitors in peripheral blood. The cells were analyzed with flow cytometry employing an anti-Tn antibody and a lectin of Vicia villosa which recognizes only the Tn determinant. In the second phase, the Tn antigen was expressed on the cultured cells from the patient on day 3 and Tn-positive cells reached 62.7% on day 9. On the other hand, Tn-positive cells were not detected in the volunteer's cultured cells. When the patient's cells were co-cultured with the cells from a healthy voluteer, the percentage of Tn-positive cells was much lower than the expected value, suggesting that the normal cells suppressed the expression of Tn antigen on the patient's cells.
| Original language | English |
|---|---|
| Pages (from-to) | 271-283 |
| Number of pages | 13 |
| Journal | The Japanese Journal of Human Genetics |
| Volume | 37 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1992 Dec 1 |
Keywords
- Tn antigen
- erythroid cells
- liquid culture
- refractory anemia
ASJC Scopus subject areas
- Genetics(clinical)
