Expression of vascular endothelial growth factor in human ocular adnexal lymphoma

Satoshi Kinoshita, Satoru Kase, Ryo Ando, Zhenyu Dong, Junichi Fukuhara, Yoko Dong, Saori Inafuku, Kousuke Noda, Mika Noda, Atsuhiro Kanda, Susumu Ishida

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


PURPOSE. To examine the expression of VEGF in extranodal marginal zone B-cell lymphoma (EMZL) and reactive lymphoid hyperplasia (RLH) of human ocular adnexa, and analyze the correlation with the intratumoral microvessel density (MVD). METHODS. Twenty-two EMZL and 16 RLH tissues were examined in this study. Paraformaldehyde- fixed, paraffin-embedded tissue sections were processed for immunohistochemistry with antibodies against VEGF and CD20. Vascular endothelial growth factor expression was analyzed using the ELISA and RT-PCR in the EMZL tissues. Microvessel density was determined based on the immunoreactivity for anti-CD34 antibody. RESULTS. Vascular endothelial growth factor immunoreactivity was detected in the cytoplasm of lymphoid cells in EMZL and RLH. ELISA and RT-PCR confirmed VEGF protein and mRNA expressions in the EMZL tissue, respectively. Vascular endothelial growth factor-immunopositive rate in B-cells was significantly higher in 12 conjunctival EMZLs than four RLHs (P < 0.01) and 10 orbital EMZLs than 12 RLHs (P < 0.05). The MVD showed a significant positive correlation with the VEGF-immunopositive rate in conjunctival and orbital EMZLs. CONCLUSIONS. This study demonstrated increased VEGF expression in human conjunctival and orbital EMZL compared with that in RLH, suggesting that VEGF plays a significant role in the pathogenesis and tumor angiogenesis of ocular adnexal lymphoma.

Original languageEnglish
Pages (from-to)3461-3467
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Issue number6
Publication statusPublished - 2014 May 13
Externally publishedYes


  • Angiogenesis
  • Conjunctival neoplasm
  • Marginal zone b-cell lymphoma
  • Orbital neoplasm
  • Reactive lymphoid hyperplasia
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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