Extraneuronal localization of acetylcholine and its release upon nicotinic stimulation in rabbits

Koichiro Kawashima; Hisayo Oohata; Kazuko Fujimoto; Takeshi Suzuki

doi:10.1016/0304-3940(89)90599-5

Extraneuronal localization of acetylcholine and its release upon nicotinic stimulation in rabbits

Koichiro Kawashima, Hisayo Oohata, Kazuko Fujimoto, Takeshi Suzuki

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

A study was undertaken to examine the origin of plasma acetylcholine (ACh). The ACh content of blood cells was about 25 times higher than that of plasma in normal rabbits (3,722 vs 140 pg/ml blood, n = 7). Plasma ACh content in rabbits having antibody against ACh was about 80 times higher than in normal rabbits, while no difference was observed in the ACh content of blood cells between the groups. Nicotine (100 μg/kg, i.v.) produced a significant increase in plasma ACh content and a decrease in the ACh content of blood cells in normal rabbits. These data demonstrate that a large amount of ACh is localized in blood cells and that a considerable proportion of plasma ACh originates from blood cells, suggesting that ACh acts not only as a neurotransmitter but also as an autacoid.

Original language	English
Pages (from-to)	336-339
Number of pages	4
Journal	Neuroscience Letters
Volume	104
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(89)90599-5
Publication status	Published - 1989 Oct 9

Keywords

Acetylcholine content
Acetylcholine release
Blood cell
Nicotine
Plasma
Radioimmunoassay

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0304-3940(89)90599-5

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title = "Extraneuronal localization of acetylcholine and its release upon nicotinic stimulation in rabbits",

abstract = "A study was undertaken to examine the origin of plasma acetylcholine (ACh). The ACh content of blood cells was about 25 times higher than that of plasma in normal rabbits (3,722 vs 140 pg/ml blood, n = 7). Plasma ACh content in rabbits having antibody against ACh was about 80 times higher than in normal rabbits, while no difference was observed in the ACh content of blood cells between the groups. Nicotine (100 μg/kg, i.v.) produced a significant increase in plasma ACh content and a decrease in the ACh content of blood cells in normal rabbits. These data demonstrate that a large amount of ACh is localized in blood cells and that a considerable proportion of plasma ACh originates from blood cells, suggesting that ACh acts not only as a neurotransmitter but also as an autacoid.",

keywords = "Acetylcholine content, Acetylcholine release, Blood cell, Nicotine, Plasma, Radioimmunoassay",

author = "Koichiro Kawashima and Hisayo Oohata and Kazuko Fujimoto and Takeshi Suzuki",

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doi = "10.1016/0304-3940(89)90599-5",

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AU - Kawashima, Koichiro

AU - Oohata, Hisayo

AU - Fujimoto, Kazuko

AU - Suzuki, Takeshi

N1 - Funding Information: This work was supporteidn part by Grant-in-Aidfo r ScientificR esearch0 1571223 from the Ministryo f EducationS, ciencea ndCulture.

PY - 1989/10/9

Y1 - 1989/10/9

N2 - A study was undertaken to examine the origin of plasma acetylcholine (ACh). The ACh content of blood cells was about 25 times higher than that of plasma in normal rabbits (3,722 vs 140 pg/ml blood, n = 7). Plasma ACh content in rabbits having antibody against ACh was about 80 times higher than in normal rabbits, while no difference was observed in the ACh content of blood cells between the groups. Nicotine (100 μg/kg, i.v.) produced a significant increase in plasma ACh content and a decrease in the ACh content of blood cells in normal rabbits. These data demonstrate that a large amount of ACh is localized in blood cells and that a considerable proportion of plasma ACh originates from blood cells, suggesting that ACh acts not only as a neurotransmitter but also as an autacoid.

AB - A study was undertaken to examine the origin of plasma acetylcholine (ACh). The ACh content of blood cells was about 25 times higher than that of plasma in normal rabbits (3,722 vs 140 pg/ml blood, n = 7). Plasma ACh content in rabbits having antibody against ACh was about 80 times higher than in normal rabbits, while no difference was observed in the ACh content of blood cells between the groups. Nicotine (100 μg/kg, i.v.) produced a significant increase in plasma ACh content and a decrease in the ACh content of blood cells in normal rabbits. These data demonstrate that a large amount of ACh is localized in blood cells and that a considerable proportion of plasma ACh originates from blood cells, suggesting that ACh acts not only as a neurotransmitter but also as an autacoid.

KW - Acetylcholine content

KW - Acetylcholine release

KW - Blood cell

KW - Nicotine

KW - Plasma

KW - Radioimmunoassay

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Extraneuronal localization of acetylcholine and its release upon nicotinic stimulation in rabbits

Abstract

Keywords

ASJC Scopus subject areas

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