Fabric reinforced polyacrylamide gels for electroblotting

Hideyuki Nishizawa; Ayako Murakami; Naoko Hayashi; Mami Iida; Yoshihiro Abe

doi:10.1002/elps.1150060708

Fabric reinforced polyacrylamide gels for electroblotting

Hideyuki Nishizawa, Ayako Murakami, Naoko Hayashi, Mami Iida, Yoshihiro Abe

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The preparation of 0.5 mm fabric reinforced polyacrylamide (FRP) gels is described. After isoelectric focusing, egg white and isoelectric point marker proteins were quantitatively transferred in only 20 min from the FRP gels to nitrocellulose membranes by electroblotting. FRP gels combine the advantages of previously described supports for polyacrylamide gels with penetrability for electric current during blotting. The gels may be conveniently stored and afford operational versatility.

Original language	English
Pages (from-to)	349-350
Number of pages	2
Journal	ELECTROPHORESIS
Volume	6
Issue number	7
DOIs	https://doi.org/10.1002/elps.1150060708
Publication status	Published - 1985

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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10.1002/elps.1150060708

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abstract = "The preparation of 0.5 mm fabric reinforced polyacrylamide (FRP) gels is described. After isoelectric focusing, egg white and isoelectric point marker proteins were quantitatively transferred in only 20 min from the FRP gels to nitrocellulose membranes by electroblotting. FRP gels combine the advantages of previously described supports for polyacrylamide gels with penetrability for electric current during blotting. The gels may be conveniently stored and afford operational versatility.",

author = "Hideyuki Nishizawa and Ayako Murakami and Naoko Hayashi and Mami Iida and Yoshihiro Abe",

year = "1985",

doi = "10.1002/elps.1150060708",

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T1 - Fabric reinforced polyacrylamide gels for electroblotting

AU - Nishizawa, Hideyuki

AU - Murakami, Ayako

AU - Hayashi, Naoko

AU - Iida, Mami

AU - Abe, Yoshihiro

PY - 1985

Y1 - 1985

N2 - The preparation of 0.5 mm fabric reinforced polyacrylamide (FRP) gels is described. After isoelectric focusing, egg white and isoelectric point marker proteins were quantitatively transferred in only 20 min from the FRP gels to nitrocellulose membranes by electroblotting. FRP gels combine the advantages of previously described supports for polyacrylamide gels with penetrability for electric current during blotting. The gels may be conveniently stored and afford operational versatility.

AB - The preparation of 0.5 mm fabric reinforced polyacrylamide (FRP) gels is described. After isoelectric focusing, egg white and isoelectric point marker proteins were quantitatively transferred in only 20 min from the FRP gels to nitrocellulose membranes by electroblotting. FRP gels combine the advantages of previously described supports for polyacrylamide gels with penetrability for electric current during blotting. The gels may be conveniently stored and afford operational versatility.

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ER -

Fabric reinforced polyacrylamide gels for electroblotting

Abstract

ASJC Scopus subject areas

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