TY - GEN
T1 - Fabrication of transplantable 3D-neuronal network
AU - Kato-Negishi, Midori
AU - Tsuda, Yukiko
AU - Onoe, Hiroaki
AU - Takeuchi, Shoji
PY - 2010/12/1
Y1 - 2010/12/1
N2 - This paper describes a method to fabricate a transplantable three-dimensional (3D) neuronal network using the neu-rospheroid network (NSN) on a polydimethylsiloxane (PDMS) microchamber array. We developed a technique, termed the "NSN-stamping method", to transfer the NSN onto other substrates without any chemical treatment. Using this method, we demonstrate that different NSNs were stacked and formed 3D neuronal tissue, and then 3D-NSN could be transferred onto the cortical tissue at a high transfer rate. This method would be an extremely effective tool for tissue buildup in neuronal tissue engineering and provides a simple approach to the transplantation of 3D neuronal network.
AB - This paper describes a method to fabricate a transplantable three-dimensional (3D) neuronal network using the neu-rospheroid network (NSN) on a polydimethylsiloxane (PDMS) microchamber array. We developed a technique, termed the "NSN-stamping method", to transfer the NSN onto other substrates without any chemical treatment. Using this method, we demonstrate that different NSNs were stacked and formed 3D neuronal tissue, and then 3D-NSN could be transferred onto the cortical tissue at a high transfer rate. This method would be an extremely effective tool for tissue buildup in neuronal tissue engineering and provides a simple approach to the transplantation of 3D neuronal network.
KW - Neuron
KW - Neuronal network
KW - Tissue engineering
KW - Transplantation
UR - http://www.scopus.com/inward/record.url?scp=84884323122&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884323122&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84884323122
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 632
EP - 634
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -