Far rapid synthesis of giant DNA in the Bacillus subtilis genome by a conjugation transfer system

Mitsuhiro Itaya; Mitsuru Sato; Miki Hasegawa; Nobuaki Kono; Masaru Tomita; Shinya Kaneko

doi:10.1038/s41598-018-26987-0

Far rapid synthesis of giant DNA in the Bacillus subtilis genome by a conjugation transfer system

Mitsuhiro Itaya, Mitsuru Sato, Miki Hasegawa, Nobuaki Kono, Masaru Tomita, Shinya Kaneko

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Bacillus subtilis offers a platform for giant DNA synthesis, which is mediated by the connection of overlapping DNA segments called domino DNA, in the cloning locus of the host. The domino method was successfully used to produce DNA fragments as large as 3500 kbp. However, domino DNA is limited to <100 kbp because of size restrictions regarding the transformation (TF) of B. subtilis competent cells. A novel conjugal transfer (CT) method was designed to eliminate the TF size limit. The CT method enables rapid and efficient domino reactions in addition to the transfer of giant DNA molecules of up to 875 kbp to another B. subtilis genome within 4 hours. The combined use of the TF and CT should enable significantly rapid giant DNA production.

Original language	English
Article number	8792
Journal	Scientific reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-26987-0
Publication status	Published - 2018 Dec 1

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abstract = "Bacillus subtilis offers a platform for giant DNA synthesis, which is mediated by the connection of overlapping DNA segments called domino DNA, in the cloning locus of the host. The domino method was successfully used to produce DNA fragments as large as 3500 kbp. However, domino DNA is limited to <100 kbp because of size restrictions regarding the transformation (TF) of B. subtilis competent cells. A novel conjugal transfer (CT) method was designed to eliminate the TF size limit. The CT method enables rapid and efficient domino reactions in addition to the transfer of giant DNA molecules of up to 875 kbp to another B. subtilis genome within 4 hours. The combined use of the TF and CT should enable significantly rapid giant DNA production.",

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AU - Itaya, Mitsuhiro

AU - Sato, Mitsuru

AU - Hasegawa, Miki

AU - Kono, Nobuaki

AU - Tomita, Masaru

AU - Kaneko, Shinya

PY - 2018/12/1

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Far rapid synthesis of giant DNA in the Bacillus subtilis genome by a conjugation transfer system

Abstract

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